PMID: 9420244Jan 7, 1998Paper

Mucosal and parenteral vaccination against acute and latent murine cytomegalovirus (MCMV) infection by using an attenuated MCMV mutant

Journal of Virology
M R MacDonaldH W Virgin

Abstract

We used a live attenuated murine cytomegalovirus (MCMV) mutant to analyze mechanisms of vaccination against acute and latent CMV infection. We selected MCMV mutant RV7 as a vaccine candidate since this virus grows well in tissue culture but is profoundly attenuated for growth in normal and severe combined immunodeficient (SCID) mice (V. J. Cavanaugh et al., J. Virol. 70:1365-1374, 1996). BALB/c mice were immunized twice (0 and 14 days) subcutaneously (s.c.) with tissue culture-passaged RV7 and then challenged with salivary gland-passaged wild-type MCMV (sgMCMV) intraperitoneally (i.p.) on day 28. RV7 vaccination protected mice against challenge with 10(5) PFU of sgMCMV, a dose that killed 100% of mock-vaccinated mice. RV7 vaccination reduced MCMV replication 100- to 500-fold in the spleen between 1 and 8 days after challenge. We used the capacity to control replication of MCMV in the spleen 4 days after challenge as a surrogate for protection. Protection was antigen specific and required both live RV7 and antigen-specific lymphocytes. Interestingly, RV7 was effective when administered s.c., i.p., perorally, intranasally, and intragastrically, demonstrating that attenuated CMV applied to mucosal surfaces can elicit protection ag...Continue Reading

References

Jan 1, 1978·Microbiology and Immunology·Y MinamishimaR Fukunishi
Nov 1, 1977·Proceedings of the Society for Experimental Biology and Medicine·R J Howard, H H Balfour
Jan 1, 1979·Microbiology and Immunology·Y Eizuru, Y Minamishima
Apr 1, 1992·Journal of Virology·P LucinU H Koszinowski
Nov 1, 1991·Reviews of Infectious Diseases·S E StarrS A Plotkin
Apr 1, 1989·The Journal of Experimental Medicine·S JonjićU H Koszinowski
Apr 1, 1987·The Journal of Infectious Diseases·S H ChandlerJ K McDougall
Oct 23, 1980·The New England Journal of Medicine·E S HuangR F Pass
Jan 1, 1980·Annals of the New York Academy of Sciences·E S HuangC Alford
Sep 1, 1983·The Journal of General Virology·Y Tonari, Y Minamishima
Mar 1, 1995·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·A S IstasJ A Stewart
Jan 1, 1995·The Journal of Infectious Diseases·W BrittC Kensil
Mar 1, 1995·Journal of Virology·J L Pollock, H W Virgin

❮ Previous
Next ❯

Citations

Jan 29, 2003·Journal of Virology·Scott A TibbettsHerbert W Virgin
Jun 12, 2013·Expert Review of Vaccines·Vijayendra DasariRajiv Khanna
Jul 15, 2003·Journal of Virology·Luka Cicin-SainUlrich H Koszinowski
Jul 20, 2005·Expert Review of Vaccines·Mark R Schleiss, Thomas C Heineman

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.