PMID: 11926896Apr 3, 2002Paper

Mucosal biofilm formation on middle-ear mucosa in the chinchilla model of otitis media

JAMA : the Journal of the American Medical Association
G D EhrlichJ Christopher Post

Abstract

Chronic otitis media with effusion (OME) has long been considered to be a sterile inflammatory process. The previous application of molecular diagnostic technologies to OME suggests that viable bacteria are present in complex communities known as mucosal biofilms; however, direct imaging evidence of mucosal biofilms associated with OM is lacking. To determine whether biofilm formation occurs in middle-ear mucosa in an experimental model of otitis media. A total of 48 research-grade, young adult chinchillas weighing 500 g were used for 2 series of animal experiments: one to obtain specimens for scanning electron microscopy and the other to obtain specimens for confocal laser scanning microscopy using vital dyes. In each series, 21 animals were bilaterally injected with viable Haemophilus influenzae bacteria and 1 was inoculated to account for expected mortality. Three served as negative controls. Effusions and mucosal specimens were collected from 2 infected animals that were euthanized at 3, 6, 12, and 24 hours and at days 2, 4, 5, 10, 16, and 22 after inoculation. Images were analyzed for biofilm morphology, including presence of microcolony formation and for presence of bacteria on tissue surfaces. Scanning electron microscop...Continue Reading

Citations

Feb 25, 2014·Journal of Infection and Chemotherapy : Official Journal of the Japan Society of Chemotherapy·Liang QinHiroshi Watanabe
Jul 26, 2014·International Journal of Pediatric Otorhinolaryngology·James C WangJoehassin Cordero
Oct 18, 2013·European Archives of Oto-rhino-laryngology : Official Journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : Affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery·Xingzhi GuHua Zhang
Dec 23, 2003·Infection and Immunity·W Edward SwordsJessica VonCannon
Feb 26, 2005·Infection and Immunity·Lauren O BakaletzRobert S Munson
Nov 18, 2005·Journal of Bacteriology·Christopher J Southey-PilligKarin Sauer
Mar 21, 2006·Journal of Bacteriology·Magee AllegrucciKarin Sauer
May 6, 2006·American Journal of Respiratory and Critical Care Medicine·Timothy D StarnerPaul B McCray
Jul 13, 2006·JAMA : the Journal of the American Medical Association·Luanne Hall-StoodleyJoseph E Kerschner
Jul 14, 2006·Emerging Infectious Diseases·Siobhán M O'ConnorJames M Hughes
Jul 21, 2006·BMC Microbiology·Timothy K GallaherRodrigo Aguilera
Nov 10, 2006·Journal of Clinical Microbiology·M Catherine McEllistremSaleem A Khan
Jun 15, 2007·Infection and Immunity·J Andy SchaberKendra P Rumbaugh
Jan 23, 2008·Archives of Otolaryngology--head & Neck Surgery·Matthew L UbellAmy Burrows
Dec 17, 2008·Journal of Bacteriology·David G Davies, Cláudia N H Marques
Feb 17, 2009·Journal of Bacteriology·Fernando Terán ArceRecep Avci
Dec 4, 2009·PloS One·Anna Dongari-BagtzoglouJohn Vasilakos
Nov 19, 2010·BMC Veterinary Research·Yasuko HirakawaHiromi Amao
Feb 11, 2011·Journal of Clinical Microbiology·L NisticoL Hall-Stoodley
Jun 8, 2011·Infection and Immunity·Matthew S ByrdW Edward Swords
Oct 25, 2011·BMC Pediatrics·Ruth B ThorntonPeter C Richmond
Sep 26, 2012·Medical Microbiology and Immunology·Chase WattersKendra P Rumbaugh

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