Mucosal immune response in cattle with subclinical Johne's disease

Veterinary Pathology
Douglas J WeissCleverson D Souza

Abstract

Mycobacterium avium subsp. paratuberculosis is the causative agent of Johne's disease, a chronic granulomatous enteritis of wild and domestic ruminants. During a long subclinical period, the organism persists in the intestine despite systemic cellular and humoral immune responses. To explore the mucosal immune response in Johne's disease, we isolated mononuclear leukocytes from the ileum of cows naturally infected with M. avium subsp. paratuberculosis and from cows that were not infected. We evaluated the immunophenotype of these cells and the proliferative responses after the addition of M. avium subsp. paratuberculosis sonicate or B-cell or T-cell mitogens. Although the percentage of T cells was increased in infected cows, these cells consisted mostly of memory (CD2+CD62L-) and regulatory (CD4+CD25+) T cells. Further evidence of immune hyporesponsiveness included a decrease in the percentage of T cells with an activated phenotype and a decrease in cells expressing major histocompatibility factor class II (MHC class II). Unlike the spleen, ileal lymphocytes from infected cows failed to proliferate in response to M. avium subsp. paratuberculosis sonicate. Additionally, ileal lymphocytes from infected cows proliferated poorly in...Continue Reading

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Citations

Oct 1, 2015·Animal Science Journal = Nihon Chikusan Gakkaihō·Yun-Mi KimGuk-Hyun Suh
Jan 1, 2014·Frontiers in Veterinary Science·Jonathan A RousseyPaul M Coussens
Apr 7, 2009·Comparative Immunology, Microbiology and Infectious Diseases·F DelgadoA L Pereira-Suárez
Jul 29, 2011·Clinical and Vaccine Immunology : CVI·Dairu ShuBryce M Buddle
Jul 31, 2008·Expert Review of Vaccines·Valérie Rosseels, Kris Huygen
Dec 3, 2013·European Journal of Microbiology & Immunology·Gangadhararao AppanaVilluppanoor Alwar Srinivasan
Feb 28, 2018·Infection and Immunity·Yamato SajikiKazuhiko Ohashi
Mar 26, 2016·Veterinary Immunology and Immunopathology·Jonathan A RousseyPaul M Coussens
Dec 5, 2013·Veterinary Immunology and Immunopathology·Brandon L PlattnerJesse M Hostetter

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Methods Mentioned

BETA
ELISA
PCR
light microscopy
flow cytometry
light

Software Mentioned

CELLQUEST

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