Mucosal immunisation of African green monkeys (Cercopithecus aethiops) with an attenuated parainfluenza virus expressing the SARS coronavirus spike protein for the prevention of SARS

Lancet
Alexander BukreyevPeter L Collins

Abstract

The outbreak of severe acute respiratory syndrome (SARS) in 2002 was caused by a previously unknown coronavirus-SARS coronavirus (SARS-CoV). We have developed an experimental SARS vaccine for direct immunisation of the respiratory tract, the major site of SARS- coronavirus transmission and disease. We expressed the complete SARS coronavirus envelope spike (S) protein from a recombinant attenuated parainfluenza virus (BHPIV3) that is being developed as a live attenuated, intranasal paediatric vaccine against human parainfluenza virus type 3 (HPIV3). We immunised eight African green monkeys, four with a single dose of BHPIV3/ SARS-S and four with a control, BHPIV3/Ctrl, administered via the respiratory tract. A SARS-coronavirus challenge was given to all monkeys 28 days after immunisation. Immunisation of animals with BHPIV3/SARS-S induced the production of SARS-coronavirus-neutralising serum antibodies, indicating that a systemic immune response resulted from mucosal immunisation. After challenge with SARS coronavirus, all monkeys in the control group shed SARS coronavirus, with shedding lasting 5-8 days. No viral shedding occurred in the group immunised with BHPIV3/SARS-S. A vectored mucosal vaccine expressing the SARS-coronavi...Continue Reading

References

Jan 1, 1981·Comparative Immunology, Microbiology and Infectious Diseases·R C Weiss, F W Scott
Aug 1, 1996·The Pediatric Infectious Disease Journal·R A KarronB R Murphy
Apr 12, 2003·The New England Journal of Medicine·Christian DrostenHans Wilhelm Doerr
Apr 12, 2003·The New England Journal of Medicine·Thomas G KsiazekUNKNOWN SARS Working Group
Oct 31, 2003·Nature·Byron E E MartinaAlbert D M E Osterhaus
Dec 19, 2003·The New England Journal of Medicine·Joseph S M PeirisKlaus Stöhr

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Citations

Dec 4, 2004·Nature Medicine·J S M PeirisK Y Yuen
Sep 3, 2004·Nature Reviews. Immunology·Hiroshi Kiyono, Satoshi Fukuyama
Feb 10, 2009·Nature Reviews. Microbiology·Lanying DuShibo Jiang
Jul 23, 2005·Viral Immunology·Yuxian He, Shibo Jiang
Dec 4, 2004·The Pediatric Infectious Disease Journal·Mark R Denison
Mar 1, 2007·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Guo-ping Zhao
Oct 17, 2006·Clinical Microbiology Reviews·Laura Gillim-Ross, Kanta Subbarao
Oct 16, 2007·Clinical Microbiology Reviews·Vincent C C ChengKwok Yung Yuen
Mar 23, 2012·Journal of Virology·Linda G BrockUrsula J Buchholz
Oct 17, 2006·Journal of Virology·Alexander BukreyevPeter L Collins
Dec 15, 2004·Journal of Virology·Anjeanette RobertsKanta Subbarao
Dec 13, 2005·Microbiology and Molecular Biology Reviews : MMBR·Susan R Weiss, Sonia Navas-Martin
Jan 25, 2007·Annual Review of Immunology·Jun Chen, Kanta Subbarao
Jan 22, 2005·Annual Review of Medicine·Claire M Fraser, Rino Rappuoli
Aug 24, 2005·Virology Journal·Martin J VincentStuart T Nichol
Apr 15, 2005·Emerging Infectious Diseases·Anthony S FauciGregory K Folkers
Aug 23, 2005·Emerging Infectious Diseases·Konrad StadlerKanta Subbarao
Mar 1, 2007·Emerging Infectious Diseases·Lin-Fa WangBryan T Eaton

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Methods Mentioned

BETA
PCR
electrophoresis
lavages
glycosylation
lavage

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