Mucosal Immunity and Protective Efficacy of Intranasal Inactivated Influenza Vaccine Is Improved by Chitosan Nanoparticle Delivery in Pigs

Frontiers in Immunology
Santosh DhakalGourapura J Renukaradhya

Abstract

Annually, swine influenza A virus (SwIAV) causes severe economic loss to swine industry. Currently used inactivated SwIAV vaccines administered by intramuscular injection provide homologous protection, but limited heterologous protection against constantly evolving field viruses, attributable to the induction of inadequate levels of mucosal IgA and cellular immune responses in the respiratory tract. A novel vaccine delivery platform using mucoadhesive chitosan nanoparticles (CNPs) administered through intranasal (IN) route has the potential to elicit strong mucosal and systemic immune responses in pigs. In this study, we evaluated the immune responses and cross-protective efficacy of IN chitosan encapsulated inactivated SwIAV vaccine in pigs. Killed SwIAV H1N2 (δ-lineage) antigens (KAg) were encapsulated in chitosan polymer-based nanoparticles (CNPs-KAg). The candidate vaccine was administered twice IN as mist to nursery pigs. Vaccinates and controls were then challenged with a zoonotic and virulent heterologous SwIAV H1N1 (γ-lineage). Pigs vaccinated with CNPs-KAg exhibited an enhanced IgG serum antibody and mucosal secretory IgA antibody responses in nasal swabs, bronchoalveolar lavage (BAL) fluids, and lung lysates that were...Continue Reading

References

Apr 1, 1991·Journal of Virology·K B Renegar, P A Small
Aug 1, 1994·Pharmaceutical Research·L IllumS S Davis
Jan 1, 1993·Annual Review of Immunology·M A Farrar, R D Schreiber
May 20, 1999·International Journal of Pharmaceutics·V DodaneJ R Merwin
Dec 20, 1999·Pharmaceutical Research·R Fernández-UrrusunoM J Alonso
Dec 30, 1999·Veterinary Immunology and Immunopathology·F A Zuckermann
Aug 23, 2001·Advanced Drug Delivery Reviews·L IllumS S Davis
Oct 5, 2001·Clinical Microbiology Reviews·C E Samuel
Nov 24, 2001·Advanced Drug Delivery Reviews·I M van der LubbenH E Junginger
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Jan 20, 2004·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Ana VilaMaría José Alonso
Nov 30, 2004·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Daqing WangJohn Samuel
Jun 18, 2005·International Journal of Pharmaceutics·Camilla FogedAnne Sundblad
Sep 3, 2005·Journal of Biomedical Materials Research. Part a·María P TorresSurya K Mallapragada
Feb 24, 2006·Nature Reviews. Immunology·Marian R Neutra, Pamela A Kozlowski
May 13, 2006·Journal of Biomaterials Science. Polymer Edition·Carlos E Astete, Cristina M Sabliov
Mar 28, 2008·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Olga BorgesHans E Junginger
Dec 17, 2008·Advances in Virus Research·Amy L VincentJürgen A Richt
May 30, 2009·Veterinary Microbiology·H M YassineY M Saif
Mar 30, 2010·Expert Opinion on Therapeutic Patents·Neeraj MishraSuresh P Vyas
May 19, 2011·International Journal of Nanomedicine·Jun Jie WangShu Ling Wang
Nov 30, 2011·Journal of Nanobiotechnology·Anil Mahapatro, Dinesh K Singh
Sep 15, 2012·Current Topics in Microbiology and Immunology·Kristien Van Reeth, Wenjun Ma
Feb 14, 2013·Current Topics in Microbiology and Immunology·B H Janke
Mar 26, 2013·Molecular Immunology·Elisa CrisciMaria Montoya
Apr 6, 2013·Zoonoses and Public Health·A VincentJ Ciacci-Zanella
Jul 28, 2013·International Journal of Pharmaceutics·Antonio RampinoAttilio Cesàro
Dec 18, 2013·AAPS PharmSciTech·Chompoonuch SawaengsakNuttanan Sinchaipanid

❮ Previous
Next ❯

Citations

Oct 27, 2018·Expert Review of Vaccines·Ye WangBao-Zhong Wang
Nov 7, 2019·Veterinary Research·Santosh Dhakal, Gourapura J Renukaradhya
Feb 9, 2019·Frontiers in Immunology·Soultan Al-HalifaDenis Archambault
Jun 11, 2019·Frontiers in Immunology·Anneleen M F MatthijsArtur Summerfield
Dec 3, 2020·Marine Drugs·Soojin Shim, Han Sang Yoo
Aug 30, 2020·International Journal of Pharmaceutics·Matheus Aparecido Dos Santos RamosMarlus Chorilli
Dec 8, 2020·Frontiers in Bioengineering and Biotechnology·Sankar Renu, Gourapura J Renukaradhya
Jan 8, 2021·Biotechnology Journal·Jingdi Pan, Zongqiang Cui
Jan 13, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jhanvi JhaveriAbdelwahab Omri
Oct 6, 2019·Veterinary Microbiology·S DhakalG J Renukaradhya
Feb 23, 2021·Emergent Materials·Farzan Vahedifard, Krishnan Chakravarthy
Dec 5, 2018·Developmental and Comparative Immunology·Elisa Casadei, Irene Salinas
Apr 6, 2021·Frontiers in Cellular and Infection Microbiology·Homa BoroumandHamed Mirzaei
Jul 25, 2021·Advanced Drug Delivery Reviews·Shuting S CaiKam W Leong
Jul 24, 2021·Archives of Virology·Mohamed FawzyAbdou Nagy
Jul 1, 2021·ACS Sustainable Chemistry & Engineering·Agnieszka M Jastrzębska, Alexey S Vasilchenko
Nov 6, 2018·ACS Infectious Diseases·Lei Deng, Bao-Zhong Wang
Jan 9, 2022·The AAPS Journal·Dylan A HendyKristy M Ainslie

❮ Previous
Next ❯

Datasets Mentioned

BETA
GM-CSF

Methods Mentioned

BETA
dynamic light-scattering
protein assay
ELISA
bronchoalveolar
flow cytometry
fluorescence-activated cell sorting
FACS
Reverse Transcription PCR
dynamic light scattering
bronchoalveolar lavage

Software Mentioned

GraphPad Prism
FlowJo

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.