Abstract
Mucosal surfaces are important for the induction of immunity against influenza virus. In a murine intranasal immunization model, we demonstrated that the attenuated Shigella flexneri Deltaasd strain 15D, carrying a DNA construct encoding the influenza virus hemagglutinin (HA), induces protective immunity against a lethal respiratory challenge with influenza A/WSN/33. Influenza virus-specific IFN-gamma T cells were detected among splenocytes, and anti-HA IgG was detected in serum post-immunization, albeit at low levels. Following influenza virus challenge, an accelerated anti-HA IgA antibody response was detected in bronchoalveolar lavage (BAL) washings from mice vaccinated with attenuated shigella containing the HA construct. These results suggest that S. flexneri Deltaasd strain 15D is a promising vector for mucosal DNA vaccine immunization against influenza virus and other mucosal pathogens.
References
Jun 1, 1991·Vaccine·P J SansonettiM L Bernardini
Jun 6, 1991·Nature·C K StoverG F Hatfull
Jul 1, 1986·The Journal of Infectious Diseases·P R JohnsonP F Wright
Apr 1, 1984·European Journal of Immunology·F Y LiewJ Beale
Dec 1, 1994·Seminars in Arthritis and Rheumatism·R A Hughes, A C Keat
Oct 13, 1995·Science·D R SizemoreJ C Sadoff
Mar 19, 1993·Science·J B UlmerA Friedman
Mar 1, 1996·Current Biology : CB·J HaasB Seed
Jun 1, 1997·Vaccine·D R SizemoreJ C Sadoff
Feb 12, 1998·The Journal of Experimental Medicine·M B Graham, T J Braciale
Jan 7, 1998·International Immunology·A BotC Bona
Mar 21, 2001·Vaccine·G A PolandR M Jacobson
May 31, 2002·Immunology Letters·William H VecinoGlenn J Fennelly
Jul 12, 2002·Vaccine·Jeffrey B Ulmer
Aug 21, 2002·Annals of Internal Medicine·Patrick Y LeeEric D Peterson
Nov 22, 2002·Vaccine·Tomoki YoshikawaShin ichi Tamura
Jan 9, 2003·JAMA : the Journal of the American Medical Association·William W ThompsonKeiji Fukuda
Feb 1, 2003·Vaccine·Terry NolanPaul M Mendelman
Apr 15, 2003·Journal of Virology·Marcela F PasettiMyron M Levine
Apr 23, 2003·Virology·Rick A BrightJacqueline M Katz
Jun 14, 2003·Vaccine·Ayato TakadaHiroshi Kida
Aug 22, 2003·Nucleic Acids Research·Jason D MarshallKaren L Fearon
Sep 25, 2003·Vaccine·H ChenK Subbarao
Citations
Mar 5, 2008·Gene Therapy·M D B LarsenE W F W Alton
Sep 10, 2010·Journal of Virology·Helga Hofmann-SieberRalf Wagner
May 26, 2010·Genetic Vaccines and Therapy·Babak JalilianSohkichi Matsumoto
Dec 23, 2006·Expert Review of Vaccines·Wolfgang Jechlinger
May 3, 2012·Journal of Controlled Release : Official Journal of the Controlled Release Society·Seyed Davoud JazayeriAbdul Rahman Omar
Apr 22, 2010·Vaccine·Karen A LiljebjelkeDarrell R Kapczynski
Mar 18, 2008·Vaccine·Paola BrunRiccardo Manganelli
Sep 25, 2007·Comparative Immunology, Microbiology and Infectious Diseases·Thierry van den BergMichel Bublot
Dec 20, 2005·The Journal of Pathology·Georges VassauxNick R Lemoine
Jun 1, 2005·Vaccine·Jianjun ChenZe Chen
Jun 3, 2014·Vaccine·Michela SaliRiccardo Manganelli
Aug 21, 2013·PloS One·Sarah OthmanJohn G Coote
Jun 15, 2010·PloS One·Peter T LoudonDeborah H Fuller
Jun 4, 2010·PloS One·Behazine CombadièreUlrike Blume-Peytavi
Mar 1, 2006·Health Physics·M Korshukin, R J Emery
Jul 29, 2006·FEMS Immunology and Medical Microbiology·Seong-Kug EoJeong-Gon Cho
Feb 12, 2012·World Journal of Virology·Maria TrovatoPiergiuseppe De Berardinis
Jan 29, 2010·Clinical and Vaccine Immunology : CVI·Zhiming PanXiufan Liu
Jan 11, 2007·Acta Biochimica Et Biophysica Sinica·Lingli JiangWeihuan Fang
Jun 9, 2017·Future Microbiology·Nitin Machindra KambleJohn Hwa Lee
Oct 14, 2021·PLoS Neglected Tropical Diseases·Francesco CitiuloLaura B Martin