Mucosal immunization with attenuated Shigella flexneri harboring an influenza hemagglutinin DNA vaccine protects mice against a lethal influenza challenge

Virology
William H VecinoGlenn J Fennelly

Abstract

Mucosal surfaces are important for the induction of immunity against influenza virus. In a murine intranasal immunization model, we demonstrated that the attenuated Shigella flexneri Deltaasd strain 15D, carrying a DNA construct encoding the influenza virus hemagglutinin (HA), induces protective immunity against a lethal respiratory challenge with influenza A/WSN/33. Influenza virus-specific IFN-gamma T cells were detected among splenocytes, and anti-HA IgG was detected in serum post-immunization, albeit at low levels. Following influenza virus challenge, an accelerated anti-HA IgA antibody response was detected in bronchoalveolar lavage (BAL) washings from mice vaccinated with attenuated shigella containing the HA construct. These results suggest that S. flexneri Deltaasd strain 15D is a promising vector for mucosal DNA vaccine immunization against influenza virus and other mucosal pathogens.

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Citations

Sep 10, 2010·Journal of Virology·Helga Hofmann-SieberRalf Wagner
May 26, 2010·Genetic Vaccines and Therapy·Babak JalilianSohkichi Matsumoto
Dec 23, 2006·Expert Review of Vaccines·Wolfgang Jechlinger
May 3, 2012·Journal of Controlled Release : Official Journal of the Controlled Release Society·Seyed Davoud JazayeriAbdul Rahman Omar
Sep 25, 2007·Comparative Immunology, Microbiology and Infectious Diseases·Thierry van den BergMichel Bublot
Dec 20, 2005·The Journal of Pathology·Georges VassauxNick R Lemoine
Mar 1, 2006·Health Physics·M Korshukin, R J Emery
Feb 12, 2012·World Journal of Virology·Maria TrovatoPiergiuseppe De Berardinis

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