Mucosal immunization with the Moraxella Catarrhalis porin m35 induces enhanced bacterial clearance from the lung: a possible role for opsonophagocytosis.

Frontiers in Immunology
Donna M EastonJ Kyd

Abstract

Moraxella catarrhalis is a significant cause of respiratory tract infection against which a vaccine is sought. Several outer membrane proteins are currently under investigation as potential vaccine antigens, including the porin M35. We have previously shown that the third external loop of M35 was immunodominant over the remainder of the protein for antibody produced in mice against the refolded recombinant protein. However, as this loop is predicted to fold inside the porin channel we also predicted that it would not be accessible to these antibodies when M35 is expressed on the surface of the bacteria in its native conformation. This study investigated the functional activity of antibodies against M35 and those specific for the loop 3 region of M35 in vitro and in vivo. Antisera from mice immunized with M35 or the loop 3-deletion, M35loop3(-), recombinant proteins were not bactericidal but did have enhanced opsonic activity, whereas antibodies raised against the loop 3 peptide were not opsoniszing indicating that the immunodominant loop 3 of M35 was not accessible to antibody as we had previously predicted. Mucosal immunization with M35, M35 that had an antigenically altered loop 3 [M35(ID78)] and M35loop3(-) enhanced the clea...Continue Reading

Citations

Nov 14, 2015·Expert Opinion on Therapeutic Targets·Dabin Ren, Michael E Pichichero
Apr 5, 2017·Otolaryngology--head and Neck Surgery : Official Journal of American Academy of Otolaryngology-Head and Neck Surgery·Melinda M PettigrewTimothy F Murphy
Jul 13, 2016·Journal of Proteome Research·Anita KarnerAndreas J Kungl

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Methods Mentioned

BETA
PCR
enzyme linked immunosorbent assay
ELISA
bronchoalveolar lavage
flow cytometry

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