Mucosal vaccination of mice with recombinant Proteus mirabilis structural fimbrial proteins

Microbes and Infection
Paola ScavonePablo Zunino

Abstract

Proteus mirabilis, a common cause of urinary tract infection (UTI), expresses several types of fimbria including mannose-resistant/Proteus-like fimbriae (MRP), uroepithelial cell adhesin (UCA), renamed non-agglutinating fimbriae (NAF) by some authors, and P. mirabilis fimbriae (PMF), which are potentially involved in adhesion to the uroepithelium. In this study, we immunised different groups of mice with recombinant structural subunits of these fimbriae (MrpA, UcaA and PmfA) using two mucosal routes (nasal and transurethral) and we transurethrally challenged the animals with a P. mirabilis uropathogenic isolate. Induction of specific serum and urine IgG and IgA was measured to assess the potential role of the humoral immune response in protection against experimental ascending P. mirabilis UTI. Intranasally MrpA- and UcaA-immunised mice were protected against P. mirabilis ascending UTI, since recovery of bacteria from kidneys and bladders was significantly lower than in PBS-treated mice, and both fimbrial subunits significantly induced specific serum and urine antibodies. Only MrpA and PmfA transurethrally immunised animals were protected only at the kidney level, and in this case only MrpA-immunised mice exhibited significant ...Continue Reading

References

Feb 1, 1979·Journal of Medical Microbiology·B W Senior
May 5, 1986·Journal of Molecular Biology·F W Studier, B A Moffatt
Oct 1, 1985·Analytical Biochemistry·P K SmithD C Klenk
Apr 11, 2000·Emerging Infectious Diseases·F W van GinkelJ R McGhee
Dec 2, 2000·Microbes and Infection·C CokerH L Mobley
Apr 9, 2001·Clinical Microbiology Reviews·P L OgraR C Welliver
Jul 24, 2002·International Journal of Antimicrobial Agents·Xin Li, Harry L T Mobley
Nov 29, 2002·Current Drug Targets. Infectious Disorders·M J McCluskie, R D Weeratna
May 3, 2003·FEMS Immunology and Medical Microbiology·Rafael PellegrinoPablo Zunino

❮ Previous
Next ❯

Citations

Oct 9, 2012·Nature Reviews. Microbiology·Chelsie E Armbruster, Harry L T Mobley
Jul 21, 2007·FEMS Immunology and Medical Microbiology·Sérgio P D RochaWaldir P Elias
Feb 27, 2016·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Mehri HabibiSaeid Bouzari
Feb 10, 2018·EcoSal Plus·Chelsie E ArmbrusterMelanie M Pearson
Mar 22, 2016·Microbiology Spectrum·Valerie P O'BrienScott J Hultgren
Nov 7, 2015·Microbiology Spectrum·Jessica N Schaffer, Melanie M Pearson

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bladder Carcinoma In Situ

Bladder Carcinoma In Situ is a superficial bladder cancer that occurs on the surface layer of the bladder. Discover the latest research on this precancerous condition in this feed.