MucR Is Required for Transcriptional Activation of Conserved Ion Transporters to Support Nitrogen Fixation of Sinorhizobium fredii in Soybean Nodules

Molecular Plant-microbe Interactions : MPMI
Jian JiaoChang Fu Tian

Abstract

To achieve effective symbiosis with legume, rhizobia should fine-tune their background regulation network in addition to activating key genes involved in nodulation (nod) and nitrogen fixation (nif). Here, we report that an ancestral zinc finger regulator, MucR1, other than its paralog, MucR2, carrying a frameshift mutation, is essential for supporting nitrogen fixation of Sinorhizobium fredii CCBAU45436 within soybean nodules. In contrast to the chromosomal mucR1, mucR2 is located on symbiosis plasmid, indicating its horizontal transfer potential. A MucR2 homolog lacking the frameshift mutation, such as the one from S. fredii NGR234, can complement phenotypic defects of the mucR1 mutant of CCBAU45436. RNA-seq analysis revealed that the MucR1 regulon of CCBAU45436 within nodules exhibits significant difference compared with that of free-living cells. MucR1 is required for active expression of transporters for phosphate, zinc, and elements essential for nitrogenase activity (iron, molybdenum, and sulfur) in nodules but is dispensable for transcription of key genes (nif/fix) involved in nitrogen fixation. Further reverse genetics suggests that S. fredii uses high-affinity transporters to meet the demand for zinc and phosphate wit...Continue Reading

References

Dec 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·G DittaD R Helinski
Sep 12, 1995·Proceedings of the National Academy of Sciences of the United States of America·J T SullivanC W Ronson
Apr 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·P G AgronD R Helinski
Dec 24, 1996·Proceedings of the National Academy of Sciences of the United States of America·P RocheJ Dénarié
Mar 1, 1997·Molecular Plant-microbe Interactions : MPMI·M A BittingerJ Handelsman
Jun 6, 1998·Proceedings of the National Academy of Sciences of the United States of America·J T Sullivan, C W Ronson
Jul 29, 1998·Molecular Microbiology·S I Patzer, K Hantke
Apr 3, 1999·Molecular Plant-microbe Interactions : MPMI·S G Pueppke, W J Broughton
Feb 9, 2000·Trends in Microbiology·N BouhoucheC I Kado
Mar 11, 2000·Molecular Biology and Evolution·S L Turner, J P Young
Feb 29, 2000·Journal of Bacteriology·M A Bittinger, J Handelsman
Jun 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·C P WolkJ M Panoff
Nov 14, 2001·The Journal of Biological Chemistry·Sophie MoreauDavid A Day
Nov 27, 2002·BioTechniques·Christopher J Marx, Mary E Lidstrom
Mar 6, 2004·Molecular Plant-microbe Interactions : MPMI·Anke BeckerJacques Batut
Mar 24, 2004·Nature Reviews. Microbiology·Eric Cascales, Peter J Christie
Jul 21, 2004·Nature Reviews. Microbiology·Ray Dixon, Daniel Kahn
Oct 6, 2005·Applied and Environmental Microbiology·Tzu-Chiao ChaoStefan Weidner
Feb 18, 2006·Microbial Cell Factories·Anna SkorupskaJaroslaw Król
Jul 3, 2007·Molecular Plant-microbe Interactions : MPMI·Monika Janczarek, Anna Skorupska
Oct 10, 2008·Molecular Plant-microbe Interactions : MPMI·Christelle BahlawaneAnke Becker
Apr 17, 2009·Proceedings of the National Academy of Sciences of the United States of America·Ilaria BaglivoPaolo V Pedone
Apr 21, 2009·Applied and Environmental Microbiology·Christel SchmeisserWolfgang R Streit
Jul 22, 2009·Nucleic Acids Research·Dmitri ParkhomchukAlexey Soldatov
Sep 22, 2009·Trends in Microbiology·Catherine Masson-BoivinJacques Batut
Dec 19, 2009·Proceedings of the National Academy of Sciences of the United States of America·Maritza Zavaleta-PastorOtto Geiger
Jan 20, 2010·PLoS Biology·Marta MarchettiCatherine Masson-Boivin
Feb 23, 2010·Current Opinion in Microbiology·Yi-Ju Hsieh, Barry L Wanner
Jun 3, 2010·Nature Reviews. Genetics·Christina Toft, Siv G E Andersson
Oct 13, 2010·Proceedings of the National Academy of Sciences of the United States of America·Shengming YangHongyan Zhu
Jul 26, 2011·Applied and Environmental Microbiology·Yan Ming ZhangWen Xin Chen
Mar 1, 2012·Journal of Bacteriology·Stefan WeidnerMichael Göttfert

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Citations

Jul 22, 2017·Plant, Cell & Environment·Isidro AbreuManuel González-Guerrero
Apr 11, 2018·Environmental Microbiology Reports·Magdalena KopycińskaMonika Janczarek
Apr 18, 2018·Molecular Plant-microbe Interactions : MPMI·Yue HuChang Fu Tian
Feb 9, 2019·Metallomics : Integrated Biometal Science·Isidro AbreuDaniel Raimunda
Mar 27, 2020·Genes·Ginaini Grazielli Doin de MouraDelphine Capela
Feb 15, 2019·International Journal of Molecular Sciences·Juan Carlos Crespo-RivasMaría José Soto
Jun 11, 2020·Scientific Reports·Gianluca D'AbroscaGaetano Malgieri
Dec 12, 2020·Computational and Structural Biotechnology Journal·Jian Jiao, Chang-Fu Tian
Jul 3, 2021·International Journal of Molecular Sciences·Sebastián Acosta-JuradoJosé-María Vinardell

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