MUG-Mel2, a novel highly pigmented and well characterized NRAS mutated human melanoma cell line

Scientific Reports
Beate RinnerIris Zalaudek

Abstract

NRAS mutation in melanoma has been associated with aggressive tumor biology and poor prognosis. Although targeted therapy has been tested for NRAS mutated melanoma, response rates still appear much weaker, than in BRAF mutated melanoma. While plenty of cell lines exist, however, only few melanogenic cell lines retain their in vivo characteristics. In this work we present an intensively pigmented and well-characterized cell line derived from a highly aggressive NRAS mutated cutaneous melanoma, named MUG-Mel2. We present the clinical course, unique morphology, angiogenic properties, growth characteristics using in vivo experiments and 3D cell culture, and results of the exome gene sequencing of an intensively pigmented melanogenic cell line MUG-Mel2, derived from a cutaneous metastasis of an aggressive NRAS p. Q61R mutated melanoma. Amongst several genetic alterations, mutations in GRIN2A, CREBP, PIK3C2G, ATM, and ATR were present. These mutations, known to reinforce DNA repair problems in melanoma, might serve as potential treatment targets. The aggressive and fast growing behavior in animal models and the obtained phenotype in 3D culture reveal a perfect model for research in the field of NRAS mutated melanoma.

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Citations

Nov 2, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Christin DurchscheinNadine Kretschmer
Jan 22, 2020·Journal of Natural Products·Alexander StallingerBeate Rinner
Jul 7, 2021·Alternatives to Laboratory Animals : ATLA·Bhumika SinghJohn Malcolm Wilkinson

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Methods Mentioned

BETA
biopsy
exome sequencing
xenograft
dissection
PCR
Assay
FACS
Protein Assay

Software Mentioned

RTCA
Ingenuity Pathway Analysis
ABI Genemapper
Variant Studio
xCELLigence Real - Time Cellular Analysis system
ModFit
FACS DIVA
ImageLab

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