Mulberry leaf extract displays antidiabetic activity in db/db mice via Akt and AMP-activated protein kinase phosphorylation

Food & Nutrition Research
Ui-Jin BaeByung-Hyun Park

Abstract

Augmenting glucose utilization in skeletal muscle via the phosphatidylinositol-3 kinase (PI3 kinase)/protein kinase B (Akt) pathway or the adenosine monophosphate (AMP)-activated protein kinase (AMPK) pathway is necessary to regulate hyperglycemia in patients with type 2 diabetes mellitus. We investigated the effect of mulberry leaf extract (MLE) on glucose uptake in skeletal muscle cells and explored its in vivo antidiabetic potential. Male db/db mice were treated with either MLE (50 mg/kg, 100 mg/kg, and 250 mg/kg) or metformin (100 mg/kg) for 8 weeks. MLE treatment stimulated glucose uptake, driven by enhanced translocation of glucose transporter 4 to cell membranes in L6 myotubes. These effects of MLE were synergistic with those of insulin and were abolished in the presence of PI3K inhibitor or AMPK inhibitor. In db/db mice, supplementation with MLE decreased fasting blood glucose and insulin levels and enhanced insulin sensitivity, with increases of p-Akt and p-AMPK in skeletal muscle. Moreover, MLE improved blood lipid parameters and attenuated hepatic steatosis in diabetic db/db mice. These findings suggest that MLE exerts antidiabetic activity through stimulating glucose disposal in skeletal muscle cells via the PI3K/Ak...Continue Reading

References

Jun 20, 1995·Proceedings of the National Academy of Sciences of the United States of America·S LundO Pedersen
Sep 21, 2007·Molecular and Cellular Endocrinology·Eun-Kyung KimByung-Hyun Park
Jul 29, 2008·Current Genomics·Feng-Qi Zhao, Aileen F Keating
Dec 23, 2008·Journal of Ethnopharmacology·Xiao MaTatsuya Hayashi
Sep 19, 2009·Journal of Agricultural and Food Chemistry·Kuei-Chuan ChanChau-Jong Wang
Sep 8, 2010·Current Opinion in Pharmacology·Roger H F Wong, Hei Sook Sul
Jan 21, 2012·Nutrition Research·Jarinyaporn NaowabootUpa Kukongviriyapan
Feb 3, 2012·The American Journal of Chinese Medicine·Jarinyaporn NaowabootArunporn Itharat
Sep 13, 2015·Molecular and Cellular Endocrinology·Revathy CarnagarinCrispin R Dass
Dec 23, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Qingpu LiuGuoping Peng
Feb 7, 2016·Chinese Journal of Natural Medicines·Eric Wei-Chiang ChanSiu-Kuin Wong
Jun 18, 2017·Complementary Therapies in Medicine·Daniel M RicheWarren L May

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Methods Mentioned

BETA
blood drawn
enzyme-linked immunosorbent assay
electrophoresis

Software Mentioned

Waters UNIFI
GraphPad Prism

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