MuLK, a eukaryotic multi-substrate lipid kinase.

The Journal of Biological Chemistry
David W WaggonerSandra Bajjalieh

Abstract

We report the identification and characterization of a novel lipid kinase that phosphorylates multiple substrates. This enzyme, which we term MuLK for multi-substrate lipid kinase, does not belong to a previously described lipid kinase family. MuLK has orthologs in many organisms and is broadly expressed in human tissues. Although predicted to be a soluble protein, MuLK co-fractionates with membranes and localizes to an internal membrane compartment. Recombinant MuLK phosphorylates diacylglycerol, ceramide, and 1-acylglycerol but not sphingosine. Although its affinity for diacylglycerol and ceramide are similar, MuLK exhibits a higher V(max) toward diacylglycerol in vitro, consistent with it acting primarily as a diacylglycerol kinase. MuLK activity was inhibited by sphingosine and enhanced by cardiolipin. It was stimulated by calcium when magnesium concentrations were low and inhibited by calcium when magnesium concentrations were high. The effects of charged lipids and cations on MuLK activity in vitro suggest that its activity in vivo is tightly regulated by cellular conditions.

References

Mar 1, 1989·The Biochemical Journal·H KanohT Imaizumi
Aug 11, 1995·The Journal of Biological Chemistry·G M CarmanE A Dennis
Jan 1, 1996·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·D N BrindleyD W Waggoner
Jan 1, 1997·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·S GhoshR M Bell
Dec 5, 1998·The Journal of Biological Chemistry·V T Hinkovska-GalchevaJ A Shayman
Apr 17, 1999·The Journal of Biological Chemistry·M K Topham, S M Prescott
Jul 30, 1999·Biochimica Et Biophysica Acta·D W WaggonerD N Brindley
Nov 24, 1999·Methods in Enzymology·S Bajjalieh, R Batchelor
Nov 18, 2000·Cellular Signalling·W J van Blitterswijk, B Houssa
Jan 5, 2002·Analytical Biochemistry·A BielawskaY A Hannun
Apr 17, 2002·The Journal of Biological Chemistry·Masako SugiuraTakafumi Kohama
Jul 10, 2002·Progress in Nucleic Acid Research and Molecular Biology·Hong LiuSarah Spiegel
Dec 19, 2002·Neuroreport·Xiaoqin YeJerold Chun
Jul 25, 2003·Trends in Biochemical Sciences·Celine LuquainAndrew J Morris
Aug 2, 2003·Nature Reviews. Cancer·Gordon B Mills, Wouter H Moolenaar

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Citations

Aug 2, 2011·Chemical Reviews·Yulia V ShulgaRichard M Epand
Dec 30, 2008·American Journal of Physiology. Lung Cellular and Molecular Physiology·Satish KalariViswanathan Natarajan
May 17, 2013·The Journal of Clinical Investigation·Xiuting ChenLibing Song
Nov 27, 2007·FEBS Letters·John S Lyssand, Sandra M Bajjalieh
Dec 26, 2012·Molecular Genetics and Metabolism·Komudi SiriwardenaJessie M Cameron
Mar 7, 2009·Biochimica Et Biophysica Acta·Jinjin CaiMatthew K Topham
Dec 5, 2006·British Journal of Pharmacology·S VandevoordeC J Fowler
Oct 10, 2013·British Journal of Pharmacology·N MurataevaK Mackie
Mar 30, 2016·Trends in Biochemical Sciences·David R AdamsNigel J Pyne
Jan 16, 2007·Biochemical and Biophysical Research Communications·Christine GrafFrédéric Bornancin
May 26, 2012·Biochemical and Biophysical Research Communications·Amanda M GellettKevin R Lynch
Aug 2, 2014·Journal of Inherited Metabolic Disease·Johannes A Mayr
Sep 5, 2017·Metabolic Brain Disease·Sanae AllaliCatherine Sarret
Nov 17, 2005·Journal of Cellular Biochemistry·Matthew K Topham
Dec 1, 2006·The Journal of Biological Chemistry·Sarah Spiegel, Sheldon Milstien
Jul 3, 2020·The Journal of Cell Biology·Michelle Grace AcobaSteven M Claypool
Jul 29, 2006·The Biochemical Journal·Philipp RovinaFrédéric Bornancin
Feb 23, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Christine GrafFrédéric Bornancin
Oct 17, 2017·Frontiers in Cell and Developmental Biology·Jan Dudek
Oct 6, 2018·Biochemical Society Transactions·Thomas Daniel JacksonDiana Stojanovski
Sep 5, 2019·Molecular Biology of the Cell·Fenja OdendallKai Stefan Dimmer
Feb 8, 2021·Genetics, Selection, Evolution : GSE·Wei LiJie Wen
Apr 30, 2020·Biochimica Et Biophysica Acta. Molecular Basis of Disease·Edoardo BerteroJan Dudek
Aug 24, 2021·Journal of Inherited Metabolic Disease·Jan Dudek, Christoph Maack
Nov 11, 2008·Cellular Signalling·Yutong Zhao, Viswanathan Natarajan

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