Müller cells do not influence leukocyte adhesion to retinal endothelial cells

Ocular Immunology and Inflammation
Ermelindo C LealAntónio F Ambrósio

Abstract

Diabetic retinopathy is associated with inflammation. The authors investigated the influence of Müller cells on leukocyte adhesion to retinal endothelial cells. ICAM-1 levels were assessed by Western blotting and immunocytochemistry. Leukocyte adhesion was quantified using a fluorescence assay. High glucose and oxidative/nitrosative stress conditions increased ICAM-1 levels in endothelial cells and leukocyte adhesion. Under the influence of Müller cells (co-cultures/conditioned medium), the effects were comparable to those found when endothelial cells were exposed, alone, to similar conditions. These results show that Müller cells do not influence leukocyte adhesion under the in vitro conditions used in this study.

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Citations

May 18, 2019·Acta Microbiologica Et Immunologica Hungarica·Francesca LosaStefano R Del Giacco
Jul 31, 2018·Frontiers in Physiology·Ana R SantiagoRosa Fernandes

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