Multi-analyte single-membrane biosensor for the serotype-specific detection of Dengue virus

Analytical and Bioanalytical Chemistry
Natalya V ZaytsevaAntje J Baeumner

Abstract

A multi-analyte biosensor based on nucleic acid hybridization and liposome signal amplification was developed for the rapid serotype-specific detection of Dengue virus. After RNA amplification, detection of Dengue virus specific serotypes can be accomplished using a single analysis within 25 min. The multi-analyte biosensor is based on single-analyte assays (see Baeumner et al (2002) Anal Chem 74:1442-1448) developed earlier in which four analyses were required for specific serotype identification of Dengue virus samples. The multi-analyte biosensor employs generic and serotype-specific DNA probes, which hybridize with Dengue RNA that is amplified by the isothermal nucleic acid sequence based amplification (NASBA) reaction. The generic probe (reporter probe) is coupled to dye-entrapping liposomes and can hybridize to all four Dengue serotypes, while the serotype-specific probes (capture probes) are immobilized through biotin-streptavidin interaction on the surface of a polyethersulfone membrane strip in separate locations. A mixture of amplified Dengue virus RNA sequences and liposomes is applied to the membrane and allowed to migrate up along the test strip. After the liposome-target sequence complexes hybridize to the specifi...Continue Reading

Citations

Sep 13, 2011·Analytical and Bioanalytical Chemistry·John T ConnellyAntje J Baeumner
Jul 6, 2013·Biomedical Microdevices·Naga Siva Kumar GundaSushanta K Mitra
Oct 15, 2005·Clinical Microbiology Reviews·Daniel V LimMarianne F Kramer
Sep 30, 2009·PLoS Neglected Tropical Diseases·Anthony R FooksThomas Müller
Dec 14, 2011·Sensors·Sabo Wada Dutse, Nor Azah Yusof
Jun 18, 2015·Sensors·Remko van den Hurk, Stephane Evoy
Jan 26, 2010·Journal of Pharmaceutical and Biomedical Analysis·Sunita KumbhatVinod Joshi
Sep 20, 2005·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Paul T Monis, Steven Giglio
Feb 7, 2018·Frontiers in Microbiology·Raj K SinghRubén Bueno-Marí
Jul 20, 2005·Lab on a Chip·Natalya V ZaytsevaAntje J Baeumner
Oct 27, 2017·Current Medicinal Chemistry·Agnieszka ChylewskaMariusz Makowski
Oct 18, 2012·The Analyst·Qingtao Liu, Ben J Boyd
Aug 13, 2008·Analytical and Bioanalytical Chemistry·Geertruida A Posthuma-TrumpieAart van Amerongen
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Nov 6, 2018·Nano-micro Letters·Kseniya SerebrennikovaAlexander Osipov
Dec 1, 2005·Analytical Chemistry·Natalya V ZaytsevaAntje J Baeumner

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