Multi-color palette of fluorescent proteins for imaging the tumor microenvironment of orthotopic tumorgraft mouse models of clinical pancreatic cancer specimens.

Journal of Cellular Biochemistry
Atsushi SuetsuguRobert M Hoffman

Abstract

Pancreatic-cancer-patient tumor specimens were initially established subcutaneously in NOD/SCID mice immediately after surgery. The patient tumors were then harvested from NOD/SCID mice and passaged orthotopically in transgenic nude mice ubiquitously expressing red fluorescent protein (RFP). The primary patient tumors acquired RFP-expressing stroma. The RFP-expressing stroma included cancer-associated fibroblasts (CAFs) and tumor-associated macrophages (TAMs). Further passage to transgenic nude mice ubiquitously expressing green fluorescent protein (GFP) resulted in tumors that acquired GFP stroma in addition to their RFP stroma, including CAFs and TAMs as well as blood vessels. The RFP stroma persisted in the tumors growing in the GFP mice. Further passage to transgenic nude mice ubiquitously expressing cyan fluorescent protein (CFP) resulted in tumors acquiring CFP stroma in addition to persisting RFP and GFP stroma, including RFP- and GFP-expressing CAFs, TAMs and blood vessels. This model can be used to image progression of patient pancreatic tumors and to visually target stroma as well as cancer cells and to individualize patient therapy.

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Apr 6, 2011·Journal of Cellular Biochemistry·Aisada UchugonovaRobert M Hoffman

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Citations

Jun 5, 2013·Cancer Research·Despina Siolas, Gregory J Hannon
Jan 22, 2015·Expert Review of Anticancer Therapy·Peter Storz
Mar 26, 2013·Cancer Investigation·Hege Karine JacobsenPer Øyvind Enger
Feb 17, 2015·Laboratory Investigation; a Journal of Technical Methods and Pathology·Robert M Hoffman
Aug 27, 2016·Journal of Cellular Biochemistry·Robert M Hoffman
May 20, 2015·Journal of Cellular Biochemistry·Atsushi SuetsuguRobert M Hoffman
Jun 8, 2015·The Cancer Journal·Valerie S LeBleu
Nov 5, 2021·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Chintan ChawdaLaura Mezzanotte

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