Multi-dimensional mapping of pyridylamine-labeled N-linked oligosaccharides by capillary electrophoresis

Journal of Chromatography. a
L R ZieskeR A O'Neill

Abstract

A simple, sensitive and reproducible multi-dimensional capillary electrophoresis (CE) oligosaccharide mapping method is reported. The structures of 20 identified N-linked oligosaccharides have been assigned mapping positions from which co-migrating unknown oligosaccharides can be characterized. The separation protocols developed have been demonstrated to separate both charged and neutral oligosaccharides. One dimension involves electroendosmotic flow-assisted CE in a sodium acetate buffer, pH 4.0. A second dimension involves separation based on borate complexation electrophoresis in a polyethylene glycol-containing buffer. A third dimension developed specifically for neutral oligosaccharides, using a sodium phosphate buffer, pH 2.5, has been shown to resolve neutral species not able to be separated by the other two dimensions. Thus, a three-dimensional map was generated to facilitate structural characterization of these oligosaccharides.

References

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Citations

Feb 24, 2001·Electrophoresis·J C WatervalW J Underberg
Jan 29, 2005·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Kae SatoSunao Yamazaki
Jun 3, 2008·Electrophoresis·Yuanwu Bao, David S Newburg
Apr 9, 2013·Analytical Chemistry·Stefan MittermayrAndrás Guttman
Dec 16, 1998·Electrophoresis·S Suzuki, S Honda
Mar 27, 2007·Glycobiology·Gerardo Alvarez-ManillaRon Orlando
Dec 18, 2013·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Shigeo Suzuki
Aug 28, 2001·Journal of Chromatography. a·M ValcárcelA Ríos

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