PMID: 429203Jan 22, 1979Paper

Multi-element analysis of the rat hippocampus by proton induced X-ray emission spectroscopy (phosphorus, sulphur, chlorine, potassium, calcium, iron, zinc, copper, lead, bromine, and rubidium)

Histochemistry
K Kemp, G Danscher

Abstract

A technique for multi-element analysis of brain tissue by proton induced X-ray emission spectroscopy (PIXE) is described and data from analysis of fixed and unfixed samples from rat hippocampus, neocortex, amygdala, and spinal cord is presented and commented on. The atoms present in the tissue are bombarded with protons which causes the ejection of electrons from the inner shells. When the "holes" are refilled with electrons from outer shells, X-ray quanta characteristic for each element are emitted. Using a high resolution energy dispersive detector a complete X-ray spectrum of the specimen can be recorded in a single measurement. Detection limits less than or approximately 5 p.p.m. of dry matter are obtained for most elements with atomic number greater than 14 (silicon). Around 13 elements were found in concentrations above the detection limits. The grand means for non-fixed hippocampi were e.g. for Zn - 120 p.p.m.; Rb - 20 p.p.m.; Fe - 150 p.p.m.; Pb - 3 p.p.m; Ni - 5 p.p.m.

References

Aug 22, 1975·Brain Research·G DanscherE J Fjerdingstad
Sep 1, 1975·Physics in Medicine and Biology·K KempJ T Moller
Jan 1, 1971·Histochemie. Histochemistry. Histochimie·F M Haug, G Danscher
Mar 21, 1974·Zeitschrift für Zellforschung und mikroskopische Anatomie·F A Geneser-JensenG Danscher
Oct 18, 1974·Brain Research·E FjerdingstadE J Fjerdingstad
Nov 15, 1974·Brain Research·E J FjerdingstadE Fjerdingstad

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Citations

Jan 1, 1983·Anatomy and Embryology·P Szerdahelyi, P Kása
Jan 1, 1987·Histochemistry·A MolownyC Lopez-Garcia
Apr 1, 1987·Biological Trace Element Research·E T Williams
Jun 24, 1985·Brain Research·J Pérez-Clausell, G Danscher
Sep 1, 1984·Journal of Neuroscience Methods·C Lopez-GarciaF J Martinez-Guijarro
Jul 17, 1998·Journal of the Neurological Sciences·M A LovellW R Markesbery
Nov 7, 2001·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·S Y GeG S Zhong
Mar 6, 2002·Microscopy Research and Technique·Carlos López-GarcíaAsunción Molowny
Jul 7, 2012·Neuroscience Research·Binbin ZhangAryeh Routtenberg
Nov 1, 2007·Basic & Clinical Pharmacology & Toxicology·Stig S JakobsenKjeld Soballe
Feb 7, 2014·Hippocampus·Alexia DaoustEmmanuel L Barbier
Jun 1, 1985·Journal of Applied Toxicology : JAT·C Winder, N G Carmichael
Oct 19, 2000·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·G DanscherR Pamphlett
May 1, 1980·Physics in Medicine and Biology·M J Renan, S J van Rensburg
Jul 11, 2001·Journal of Neuropathology and Experimental Neurology·M StoltenbergG Danscher
Oct 1, 1982·The Journal of General Psychology·Michael D Chafetz

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