Multi-factor regulation of pectate lyase secretion by Colletotrichum gloeosporioides pathogenic on avocado fruits.

Molecular Plant Pathology
Itay MiyaraD Prusky

Abstract

Tissue alkalinization during Colletotrichum gloeosporioides attack enhances the expression of PELB, which encodes pectate lyase (PL), and PL secretion, which is considered essential for full virulence. We studied the regulation of PL secretion by manipulation of C. gloeosporioides PELB. PELB was down-regulated by knocking out PAC1, which encodes the PacC transcription factor that regulates gene products with pH-sensitive activities. We functionally characterized a PACC gene homologue, PAC1, from C. gloeosporioides wild-type (WT) Cg-14 and two independent deletion strains, Deltapac1(372)and Deltapac1(761). Loss-of-function PAC1 mutants showed 85% reduction of PELB transcript expression, delayed PL secretion and dramatically reduced virulence, as detected in infection assays with avocado fruits. In contrast, PELB was up-regulated in the presence of carbon sources such as glucose. When glucose was used as a carbon source in the medium for the WT strain and the Deltapac1 mutant at pH 6.0, PELB transcript expression and PL secretion were activated. Other sugars, such as sucrose and fructose (but not galactose), also activated PELB expression. These results suggest that the pH-regulated response is only part of a multi-factor regulat...Continue Reading

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Citations

Aug 7, 2013·Applied Microbiology and Biotechnology·Tianyuan ZhangHongye Li
Dec 20, 2012·Antioxidants & Redox Signaling·Noam AlkanDov Prusky
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Nov 26, 2021·Molecular Plant Pathology·Wenxiao JiaoMaorun Fu
Jan 7, 2022·World Journal of Microbiology & Biotechnology·Jing ZhuMingwen Zhao

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