Multi-functional roles for the polypeptide transport associated domains of Toc75 in chloroplast protein import

ELife
Yamuna D PailaDanny J Schnell

Abstract

Toc75 plays a central role in chloroplast biogenesis in plants as the membrane channel of the protein import translocon at the outer envelope of chloroplasts (TOC). Toc75 is a member of the Omp85 family of bacterial and organellar membrane insertases, characterized by N-terminal POTRA (polypeptide-transport associated) domains and C-terminal membrane-integrated β-barrels. We demonstrate that the Toc75 POTRA domains are essential for protein import and contribute to interactions with TOC receptors, thereby coupling preprotein recognition at the chloroplast surface with membrane translocation. The POTRA domains also interact with preproteins and mediate the recruitment of molecular chaperones in the intermembrane space to facilitate membrane transport. Our studies are consistent with the multi-functional roles of POTRA domains observed in other Omp85 family members and demonstrate that the domains of Toc75 have evolved unique properties specific to the acquisition of protein import during endosymbiotic evolution of the TOC system in plastids.

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Citations

Mar 12, 2017·The Journal of Biological Chemistry·Mónica ZuffereyFelix Kessler
Mar 1, 2018·Annual Review of Plant Biology·Eva C M Nowack, Andreas P M Weber
Jun 27, 2018·Photosynthesis Research·Bettina Bölter
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Nov 16, 2019·Journal of Experimental Botany·Lynn G L Richardson, Danny J Schnell
Apr 15, 2020·Journal of Phycology·Arwa GabrDebashish Bhattacharya
Mar 1, 2018·Essays in Biochemistry·Serena SchwenkertJürgen Soll
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Apr 18, 2020·Frontiers in Plant Science·Shanshan HuCheng Zhu
May 27, 2021·The Journal of Cell Biology·Yakubu Princely AbuduTerje Johansen
Jun 1, 2017·Proceedings of the National Academy of Sciences of the United States of America·Patrick K O'NeilDanny Schnell
Feb 26, 2022·The New Phytologist·Arwa GabrDebashish Bhattacharya

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Datasets Mentioned

BETA
AT3G46740

Methods Mentioned

BETA
GTPases
pull-down
PCR
transgenic
genotyping
GTPase
electrophoresis
Co-immunoprecipitation

Software Mentioned

Primer
Graphpad Prism
Realplex
ImageJ
ImageQuant TL

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