Multi-functionalized carbon dots as theranostic nanoagent for gene delivery in lung cancer therapy

Scientific Reports
Yu-Fen WuTzu-Wei Wang

Abstract

Theranostics, an integrated therapeutic and diagnostic system, can simultaneously monitor the real-time response of therapy. Different imaging modalities can combine with a variety of therapeutic moieties in theranostic nanoagents. In this study, a multi-functionalized, integrated theranostic nanoagent based on folate-conjugated reducible polyethylenimine passivated carbon dots (fc-rPEI-Cdots) is developed and characterized. These nanoagents emit visible blue photoluminescence under 360 nm excitation and can encapsulate multiple siRNAs (EGFR and cyclin B1) followed by releasing them in intracellular reductive environment. In vitro cell culture study demonstrates that fc-rPEI-Cdots is a highly biocompatible material and a good siRNA gene delivery carrier for targeted lung cancer treatment. Moreover, fc-rPEI-Cdots/pooled siRNAs can be selectively accumulated in lung cancer cells through receptor mediated endocytosis, resulting in better gene silencing and anti-cancer effect. Combining bioimaging of carbon dots, stimulus responsive property, gene silencing strategy, and active targeting motif, this multi-functionalized, integrated theranostic nanoagent may provide a useful tool and platform to benefit clinicians adjusting therapeu...Continue Reading

References

Sep 19, 1998·Journal of Controlled Release : Official Journal of the Controlled Release Society·S Wang, P S Low
Feb 13, 2001·Drug Discovery Today·C P. Leamon, P S. Low
May 24, 2005·Pharmacology & Therapeutics·Ray K M Leung, Paul A Whittaker
Jan 26, 2006·Journal of the American Chemical Society·Rui HongVincent M Rotello
Jul 25, 2007·Angewandte Chemie·Haipeng LiuChengde Mao
Oct 10, 2007·Molecular Therapy : the Journal of the American Society of Gene Therapy·Shyh-Dar LiLeaf Huang
Jan 24, 2009·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Y Y HeXiaoyuan Fan
Sep 3, 2009·Journal of the American Chemical Society·Sheng-Tao YangYa-Ping Sun
Oct 22, 2009·Small·Ayush Verma, Francesco Stellacci
May 13, 2010·Angewandte Chemie·Haitao LiShuit-Tong Lee
Aug 6, 2010·Angewandte Chemie·Sheila N Baker, Gary A Baker
Nov 17, 2010·Journal of Controlled Release : Official Journal of the Controlled Release Society·Amir K VarkouhiHidde J Haisma
Mar 24, 2011·Molecular Therapy : the Journal of the American Society of Gene Therapy·Phong TrangFrank J Slack
May 27, 2011·Accounts of Chemical Research·Rizia BardhanNaomi J Halas
Nov 1, 2011·Journal of Controlled Release : Official Journal of the Controlled Release Society·Wei XiaXiaohu Zhao
Nov 13, 2013·ACS Nano·Yan YanFrank Caruso

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Citations

Feb 9, 2018·Chemical Society Reviews·Jianliang ShenHaifa Shen
May 4, 2018·Therapeutic Delivery·Gaurav ParekhStefano Leporatti
Sep 21, 2018·Expert Opinion on Drug Delivery·Mariana MagalhãesFrancisco Veiga
Mar 16, 2019·Nanoscale·Sajini D HettiarachchiRoger M Leblanc
Apr 13, 2019·Expert Review of Molecular Diagnostics·Pravin ShendeR S Gaud
May 28, 2019·Journal of Nanobiotechnology·Monika RuzyckaIreneusz P Grudzinski
Oct 24, 2019·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Zhihang ChenZaver M Bhujwalla
Oct 24, 2019·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Novi IrmaniaJia-Yaw Chang
May 1, 2019·Cancers·Anubhab MukherjeeSudip Mukherjee
Jul 27, 2017·Scientific Reports·Rajvinder KardaSimon N Waddington
Jan 4, 2019·Analytical and Bioanalytical Chemistry·Qianqian DuanShengbo Sang
Aug 28, 2017·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Kamakshi BankotiSantanu Dhara
Sep 5, 2020·Pharmaceutics·Shi Su, Peter M Kang
Feb 16, 2017·Polymers·Yiqun ZhouRoger M Leblanc
Feb 20, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Zedi ZhangShikao Shi
Dec 28, 2016·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Kai ZhengAldo R Boccaccini
Feb 28, 2018·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Zihnil Adha Islamy MazradSung Young Park
Jun 12, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Keiko YoneyamaAkiharu Satake
Oct 28, 2019·Journal of Controlled Release : Official Journal of the Controlled Release Society·Sajini D HettiarachchiRoger M Leblanc
Mar 17, 2021·Therapeutic Delivery·Tahereh Kashkoulinejad-KouhiBlanca Arnaiz
Apr 4, 2021·Nanomaterials·Helena B A SousaJoão A V Prior
May 1, 2021·Cancers·Giuseppe NocitoSabrina Conoci
Apr 21, 2021·Chemistry : a European Journal·Kenta HagiwaraNick Serpone
Jun 18, 2021·Nano-micro Letters·Shruti Rawal, Mayur Patel
Nov 14, 2019·ACS Omega·Jonathan DanielMichel Vaultier
Jul 10, 2019·Chemical Reviews·Nishtha PanwarXiaoyuan Chen
Jan 8, 2018·ACS Biomaterials Science & Engineering·Sha LiZonghua Liu
Dec 16, 2020·ACS Biomaterials Science & Engineering·Rajendiran KeerthigaAiling Fu
Aug 28, 2021·Nanomaterials·Spyridon Gavalas, Antonios Kelarakis

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Methods Mentioned

BETA
dynamic light scattering
transmitted electron microscopy
fluorescence microscopy
electrophoresis
chemical modification
ELISA
PCR
transfection

Software Mentioned

Caliper

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