Multi-input and -output logic circuits based on bioelectrocatalysis with horseradish peroxidase and glucose oxidase immobilized in multi-responsive copolymer films on electrodes

Biosensors & Bioelectronics
Xue YuHongyun Liu

Abstract

Herein, poly(N-isopropylacrylamide-co-N,N'-dimethylaminoethylmethacrylate) copolymer films were polymerized on electrode surface with a simple one-step method, and the enzyme horseradish peroxidase (HRP) was embedded in the films simultaneously, which were designated as P(NiPAAm-co-DMEM)-HRP. The films exhibited a reversible structure change with the external stimuli, such as pH, CO2, temperature and SO4(2-), causing the cyclic voltammetric (CV) response of electroactive K3Fe(CN)6 at the film electrodes to display the corresponding multi-stimuli sensitive ON-OFF behavior. Based on the switchable CV property of the system and the electrochemical reduction of H2O2 catalyzed by HRP in the films and mediated by Fe(CN)6(3-) in solution, a 5-input/3-output logic gate was established. To further increase the complexity of the logic system, another enzyme glucose oxidase (GOD) was added into the films, designated as P(NiPAAm-co-DMEM)-HRP-GOD. In the presence of oxygen, the oxidation of glucose in the solution was catalyzed by GOD in the films, and the produced H2O2 in situ was recognized and electrocatalytically reduced by HRP and mediated by Fe(CN)6(3-). Based on the bienzyme films, a cascaded or concatenated 4-input/3-output logic ga...Continue Reading

References

Feb 3, 2007·Langmuir : the ACS Journal of Surfaces and Colloids·Caroline BaluMichel Cloitre
Mar 21, 2007·Langmuir : the ACS Journal of Surfaces and Colloids·Hitoshi OhnukiMitsuru Izumi
Jan 8, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·Xia HanYing Hu

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Citations

Feb 28, 2017·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Evgeny Katz
Jul 20, 2018·Journal of the Royal Society, Interface·Dušan PetrovićJose M Sanchez-Ruiz
Dec 3, 2016·Analytical and Bioanalytical Chemistry·Evgeny KatzMichael J Schöning

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