PMID: 2121057Sep 1, 1990Paper

Multi-laboratory evaluation of a scrub typhus diagnostic kit

The American Journal of Tropical Medicine and Hygiene
D J KellyG E Lewis

Abstract

Scrub typhus is a major cause of febrile illness throughout the Asia-Pacific region. It is commonly undiagnosed, partly because of the lack of a simple, reliable diagnostic test which can be used in clinical laboratories. The indirect immunoperoxidase technique, configured into a test kit, was provided to technicians who were trained in its use. They used the kit during a 2 year field trial in their respective clinical hospital laboratories throughout Malaysia. In an evaluation using 1,722 consecutive sera tested in those laboratories, the kit was found to have a median sensitivity for IgG detection of 0.85 (range 0.33-0.95), a median specificity of 0.94 (range 0.88-1.00), reproducibility of 0.86, and efficiency of 0.92 when compared to the reference laboratory. In a proficiency survey in which 10 laboratories received 3 coded test samples, all but 2 laboratories had results within 1 dilution of the reference laboratory in quantitating specific IgG, whereas 7 laboratories were within 1 dilution in quantitating IgM. The shelf life of the kit was at least 1 year at 4 degrees C.

Citations

May 10, 2003·Clinical and Diagnostic Laboratory Immunology·Won-Jong JangIk-Sang Kim
Jul 1, 1997·Transactions of the Royal Society of Tropical Medicine and Hygiene·J PradutkanchanaD Strickman
Mar 1, 1995·Infection·A MarschangF von Sonnenburg
Jan 1, 1997·Scandinavian Journal of Infectious Diseases·W C ChiF F Chen
Mar 25, 2015·The American Journal of Tropical Medicine and Hygiene·Wuttikon RodkvamtookWei-Mei Ching
Oct 31, 2020·Tropical Medicine and Infectious Disease·Daniel H ParisAllen L Richards
May 1, 1996·Clinics in Dermatology·J D Baxter

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