Multi-locus sequence typing of Treponema pallidum subsp. pallidum present in clinical samples from France: Infecting treponemes are genetically diverse and belong to 18 allelic profiles

PloS One
Petra PospíšilováDavid Smajs

Abstract

Treponema pallidum subsp. pallidum, the causative agent of sexually transmitted syphilis, detected in clinical samples from France, was subjected to molecular typing using the recently developed Multilocus Sequence Typing system. The samples (n = 133) used in this study were collected from 2010-2016 from patients with diagnosed primary or secondary syphilis attending outpatient centers or hospitals in several locations in France. Altogether, 18 different allelic profiles were found among the fully typed samples (n = 112). There were five allelic variants identified for TP0136, 12 for TP0548, and eight for TP0705. Out of the identified alleles, one, seven, and three novel alleles were identified in TP0136, TP0548, and TP0705, respectively. Partial allelic profiles were obtained from 6 samples. The majority of samples (n = 110) belonged to the SS14-like cluster of TPA isolates while 7 clustered with Nichols-like isolates. Patients infected with Nichols-like samples were more often older (p = 0.041) and more often diagnosed with secondary syphilis (p = 0.033) compared to patients infected with SS14-like samples. In addition, macrolide resistance caused by the A2058G mutation was found to be associated with allelic profile 1.3.1 or...Continue Reading

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Citations

Oct 17, 2018·The Journal of Infectious Diseases·Linda GrillováDavid Šmajs
Oct 24, 2020·Journal of the European Academy of Dermatology and Venereology : JEADV·M JanierR Patel
Jun 21, 2021·Survey of Ophthalmology·João M FurtadoJustine R Smith
Aug 21, 2021·Future Microbiology·Candela Fernández-NavalJuliana Esperalba
Nov 19, 2021·Molecular Biology and Evolution·Marta Pla-DíazFernando González-Candelas

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Datasets Mentioned

BETA
MH105906
MH105907-13
MH105914-16
CP004010.2

Methods Mentioned

BETA
PCR
ELISA
biopsy

Software Mentioned

Lasergene
ECDCT
Network
DNASTAR
Sequence Matrix
STATISTICA
MEGA
MLST

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