Multi-matrix, dual polarity, tandem mass spectrometry imaging strategy applied to a germinated maize seed: toward mass spectrometry imaging of an untargeted metabolome

The Analyst
Adam D FeenstraYoung Jin Lee

Abstract

Mass spectrometry imaging (MSI) provides high spatial resolution information that is unprecedented in traditional metabolomics analyses; however, the molecular coverage is often limited to a handful of compounds and is insufficient to understand overall metabolomic changes of a biological system. Here, we propose an MSI methodology to increase the diversity of chemical compounds that can be imaged and identified, in order to eventually perform untargeted metabolomic analysis using MSI. In this approach, we use the desorption/ionization bias of various matrixes for different metabolite classes along with dual polarities and a tandem MSI strategy. The use of multiple matrixes and dual polarities allows us to visualize various classes of compounds, while data-dependent MS/MS spectra acquired in the same MSI scans allow us to identify the compounds directly on the tissue. In a proof of concept application to a germinated corn seed, a total of 166 unique ions were determined to have high-quality MS/MS spectra, without counting structural isomers, of which 52 were identified as unique compounds. According to an estimation based on precursor MSI datasets, we expect over five hundred metabolites could be potentially identified and visu...Continue Reading

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Citations

May 9, 2016·Trends in Plant Science·Yonghui DongAsaph Aharoni
Jun 29, 2016·Journal of the American Society for Mass Spectrometry·Maria Emilia DueñasYoung Jin Lee
May 11, 2017·Wiley Interdisciplinary Reviews. Systems Biology and Medicine·Ying-Ning HoYu-Liang Yang
Jul 8, 2017·Chemical Record : an Official Publication of the Chemical Society of Japan ... [et Al.]·Rebecca L Hansen, Young Jin Lee
Oct 20, 2018·The Analyst·Lei YinJingkai Gu
Mar 20, 2019·Scientific Reports·Yogini JaiswalLeonard Williams
Oct 31, 2020·Analytical and Bioanalytical Chemistry·Wendy H MüllerGauthier Eppe
Dec 20, 2020·Journal of Chromatography. a·Hua LiGuowang Xu
Mar 6, 2019·Journal of the American Society for Mass Spectrometry·Isaac K AttahRichard D Smith
Jan 5, 2017·Journal of the American Society for Mass Spectrometry·Adam D FeenstraYoung Jin Lee
May 12, 2017·Journal of the American Society for Mass Spectrometry·Rebecca L Hansen, Young Jin Lee
Jul 31, 2021·Plant Communications·David B MedeirosAlisdair R Fernie
Dec 22, 2016·Accounts of Chemical Research·Sage J B DunhamJonathan V Sweedler

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