Multi-metal-dependent nucleic acid enzymes

Metallomics : Integrated Biometal Science
Wenhu Zhou, Juewen Liu

Abstract

Nucleic acid enzymes (NAEs) are catalytically active RNA and DNA molecules. NAEs with RNA-cleaving activity are most extensively studied for applications in analytical chemistry, gene therapy and nanotechnology. Most NAEs require metal ions for activity. From a biochemical standpoint, these NAEs are reminiscent of metalloprotein enzymes with metal binding sites. While most NAEs require a single metal for the reaction, more and more recent examples have emerged that use two or even three metals for the reaction. The metal binding profile is sharper for these NAEs if they use the same metal ion due to cooperativity. Detailed studies have indicated examples of lanthanide and Ca2+ binding DNAzymes, where the metals interact with the non-bridging oxygen atoms in the scissile phosphate, and these DNAzymes often have a very strong thio effect that cannot be rescued by adding thiophilic metals. Another type uses multiple different metals, where one metal interacts with the scissile phosphate and the other binds to the catalytic loop for allosteric interactions. Such allosteric NAEs can also be obtained via rational design or intentional selection based on existing NAEs. These multi-metal NAEs might be useful as logic gates with metal i...Continue Reading

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Citations

Jul 11, 2018·Chembiochem : a European Journal of Chemical Biology·Lingzi Ma, Juewen Liu
Nov 7, 2018·Chembiochem : a European Journal of Chemical Biology·Yanping HeJuewen Liu
Apr 17, 2019·Chembiochem : a European Journal of Chemical Biology·Yanping HeJuewen Liu
Dec 24, 2019·Angewandte Chemie·Po-Jung Jimmy HuangJuewen Liu
Apr 26, 2020·Dalton Transactions : an International Journal of Inorganic Chemistry·Giuseppe CassoneClaudia Foti
Oct 28, 2017·Chembiochem : a European Journal of Chemical Biology·Tianmeng YuJuewen Liu
Nov 17, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Fengjie YangQiang Zhang
Jan 25, 2021·Analytica Chimica Acta·Yongjie YangJuewen Liu
Oct 27, 2020·ChemistryOpen·Po-Jung Jimmy Huang, Juewen Liu
Oct 11, 2020·Bioorganic & Medicinal Chemistry·Yang LiJunlin He
Aug 3, 2021·Journal of Chemical Theory and Computation·Briana T A BoychukStacey D Wetmore
Oct 3, 2018·Biochemistry·Po-Jung Jimmy HuangJuewen Liu
Oct 5, 2021·Journal of the American Chemical Society·Tianqing Zhang, Bryan Wei

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Methods Mentioned

BETA
biosensor
biosensors
X-ray
PCR
electrophoresis
in vitro transcription
footprinting
Fluorescence
fluorescence resonance energy transfer
isothermal

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