Multi-modal imaging of long-term recovery post-stroke by positron emission tomography and matrix-assisted laser desorption/ionisation mass spectrometry

Rapid Communications in Mass Spectrometry : RCM
Fiona HendersonAdam McMahon

Abstract

Stroke is a leading cause of disability worldwide. Understanding the recovery process post-stroke is essential; however, longer-term recovery studies are lacking. In vivo positron emission tomography (PET) can image biological recovery processes, but is limited by spatial resolution and its targeted nature. Untargeted mass spectrometry imaging offers high spatial resolution, providing an ideal ex vivo tool for brain recovery imaging. Magnetic resonance imaging (MRI) was used to image a rat brain 48 h after ischaemic stroke to locate the infarcted regions of the brain. PET was carried out 3 months post-stroke using the tracers [18 F]DPA-714 for TSPO and [18 F]IAM6067 for sigma-1 receptors to image neuroinflammation and neurodegeneration, respectively. The rat brain was flash-frozen immediately after PET scanning, and sectioned for matrix-assisted laser desorption/ionisation mass spectrometry (MALDI-MS) imaging. Three months post-stroke, PET imaging shows minimal detection of neurodegeneration and neuroinflammation, indicating that the brain has stabilised. However, MALDI-MS images reveal distinct differences in lipid distributions (e.g. phosphatidylcholine and sphingomyelin) between the scar and the healthy brain, suggesting tha...Continue Reading

References

Jan 1, 1983·Journal of Neurology, Neurosurgery, and Psychiatry·C E SkilbeckV A Wood
Jul 1, 1994·Stroke; a Journal of Cerebral Circulation·T LiuG Z Feuerstein
Sep 1, 1996·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·S A Loddick, N J Rothwell
Aug 13, 1998·International Immunology·S Fuller-EspieL M Spain
Oct 3, 1999·Physiological Reviews·P Lipton
Mar 20, 2001·Nature Reviews. Molecular Cell Biology·M P Mattson
Jan 18, 2003·Biochimica Et Biophysica Acta·Benjamin J PettusYusuf A Hannun
Sep 30, 2003·Critical Reviews in Neurobiology·John W Phillis, Michael H O'Regan
Oct 29, 2003·Antioxidants & Redox Signaling·Rao Muralikrishna AdibhatlaRobert J Dempsey
Mar 17, 2004·The Journal of Physiology·Tom SchillingClaudia Eder
May 23, 2006·Current Opinion in Neurobiology·Tadeusz Wieloch, Karoly Nikolich
Nov 14, 2006·Nucleic Acids Research·Manish SudShankar Subramaniam
Dec 5, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Christian StockClaudia Eder
Mar 29, 2007·Cerebral Cortex·Isabelle LoubinouxFrançois Chollet
Jan 15, 2008·Journal of Neuroimaging : Official Journal of the American Society of Neuroimaging·Venkatakrishna RajajeeUNKNOWN UCLA MRI Acute Stroke Investigators
Apr 17, 2008·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Michelle L JamesMichael Kassiou
Jul 9, 2008·Current Opinion in Neurology·Carolina CiumasPhilippe Ryvlin
Nov 26, 2008·Neurorehabilitation and Neural Repair·Amy BrodtmannGeoffrey Donnan
Oct 2, 2009·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Abraham MartínBertrand Tavitian
Feb 24, 2010·Circulation·Donald Lloyd-JonesUNKNOWN American Heart Association Statistics Committee and Stroke Statistics Subcommittee
Apr 13, 2010·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Abraham MartínBertrand Tavitian
Apr 20, 2010·Future Neurology·Jonathan R WeinsteinThomas Möller
Dec 2, 2010·Nature Reviews. Drug Discovery·Rainer RupprechtMichael Schumacher
Jan 27, 2011·Journal of Lipid Research·Hay-Yan J WangHsuan-Wen Wu
Mar 2, 2011·Brain, Behavior, and Immunity·Caroline DrakeStuart M Allan
Sep 29, 2011·Journal of the American Society for Mass Spectrometry·Joseph A HankinRobert C Murphy
Dec 18, 2013·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Michelle L JamesFrederick T Chin
Oct 30, 2014·European Journal of Nuclear Medicine and Molecular Imaging·Hervé BoutinWilliam Trigg
Jan 21, 2015·Nature Chemical Biology·Hee-Yong Kim
Feb 24, 2015·Journal of Pharmacological Sciences·Teruo Hayashi
Feb 14, 2016·Journal of Neuroscience Methods·Aurelie RouxAmina S Woods

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Citations

Nov 6, 2018·The Journal of Comparative Neurology·Guang Xu, Jianjun Li
Mar 5, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Christos TapeinosGianni Ciofani
May 29, 2021·RSC Medicinal Chemistry·Hebaalla Agha, Christopher R McCurdy
Jul 11, 2021·European Journal of Nuclear Medicine and Molecular Imaging·Nadja Van CampHervé Boutin
Aug 5, 2021·Metabolic Brain Disease·Laura K SchnackenbergE Ellen Jones

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