Multi-omic Directed Discovery of Cellulosomes, Polysaccharide Utilization Loci, and Lignocellulases from an Enriched Rumen Anaerobic Consortium

Applied and Environmental Microbiology
Geizecler TomazettoFabio M Squina

Abstract

Lignocellulose is one of the most abundant renewable carbon sources, representing an alternative to petroleum for the production of fuel and chemicals. Nonetheless, the lignocellulose saccharification process, to release sugars for downstream applications, is one of the most crucial factors economically challenging to its use. The synergism required among the various carbohydrate-active enzymes (CAZymes) for efficient lignocellulose breakdown is often not satisfactorily achieved with an enzyme mixture from a single strain. To overcome this challenge, enrichment strategies can be applied to develop microbial communities with an efficient CAZyme arsenal, incorporating complementary and synergistic properties, to improve lignocellulose deconstruction. We report a comprehensive and deep analysis of an enriched rumen anaerobic consortium (ERAC) established on sugarcane bagasse (SB). The lignocellulolytic abilities of the ERAC were confirmed by analyzing the depolymerization of bagasse by scanning electron microscopy, enzymatic assays, and mass spectrometry. Taxonomic analysis based on 16S rRNA sequencing elucidated the community enrichment process, which was marked by a higher abundance of Firmicutes and Synergistetes species. Shotg...Continue Reading

References

Jan 27, 1999·Bioinformatics·S R Eddy
Jan 1, 1948·The Biochemical Journal·E I McDougall
Oct 8, 2008·Nucleic Acids Research·Brandi L CantarelBernard Henrissat
Dec 25, 2008·Chemical Record : an Official Publication of the Chemical Society of Japan ... [et Al.]·Edward A BayerHarry J Flint
May 22, 2009·BMC Bioinformatics·Jochen BlomAlexander Goesmann
Jun 11, 2010·Proceedings of the National Academy of Sciences of the United States of America·J Gregory CaporasoRob Knight
Sep 10, 2011·Bioinformatics·Tanja Magoč, Steven L Salzberg
Oct 1, 2011·Nature Methods·Thomas Nordahl PetersenHenrik Nielsen
Mar 6, 2012·Nature Methods·Ben Langmead, Steven L Salzberg
May 31, 2012·Nucleic Acids Research·Yanbin YinYing Xu
Dec 4, 2012·Nature Methods·Nicholas A BokulichJ Gregory Caporaso
Dec 12, 2012·PloS One·Daniela A RibeiroFabio M Squina
Mar 23, 2013·Molecular Biology and Evolution·Zhang Wang, Martin Wu
Sep 26, 2013·Frontiers in Microbiology·Kristen M DeangelisErrol W Robinson
Mar 4, 2014·Genome Biology·Derrick E Wood, Steven L Salzberg
Mar 20, 2014·Bioinformatics·Torsten Seemann
Apr 4, 2014·Bioinformatics·Anthony M BolgerBjoern Usadel
Jul 6, 2014·Microbial Biotechnology·Christopher J CreeveySinead C Leahy
Jul 9, 2014·Annual Review of Microbiology·Bryan A WhiteHarry J Flint
Nov 12, 2014·Annual Review of Animal Biosciences·Stuart E Denman, Christopher S McSweeney
Apr 8, 2015·Environmental Microbiology·Yonit Ben DavidSarah Moraïs
Jun 11, 2015·Bioinformatics·Felipe A SimãoEvgeny M Zdobnov
Jan 20, 2016·Environmental Microbiology·Isaac CannRoderick I Mackie
Feb 26, 2016·Scientific Reports·Shuo JiaoGehong Wei
Mar 26, 2016·Methods : a Companion to Methods in Enzymology·Dinghua LiTak-Wah Lam
Jun 15, 2016·Proceedings of the National Academy of Sciences of the United States of America·Immacolata VendittoCarlos M G A Fontes
Jul 16, 2016·Applied Microbiology and Biotechnology·Diego Javier JiménezJan Dirk van Elsas
Aug 7, 2016·Biotechnology for Biofuels·Sarah MoraïsEdward A Bayer
Oct 30, 2016·Environmental Microbiology·Lizi BensoussanItzhak Mizrahi

❮ Previous
Next ❯

Citations

Jun 13, 2021·Enzyme and Microbial Technology·Douglas Antonio Alvaredo PaixãoFabio Marcio Squina
Jul 3, 2021·International Journal of Environmental Research and Public Health·Ogechukwu Bose ChukwumaNorli Ismail

❮ Previous
Next ❯

Datasets Mentioned

BETA
PRJEB30762

Methods Mentioned

BETA
scanning electron microscopy
PCR
DNA assay
protein assay

Software Mentioned

USEARCH
SAMtools
CAZyme
BUSCO
UCLUST EDGAR
ScaffoldQ +
SignalP
EDGAR
TMHMM
WebMGA

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.