Multi-omic network-based interrogation of rat liver metabolism following gastric bypass surgery featuring SWATH proteomics

Technology
Gautham Vivek SridharanNima Saeidi

Abstract

Morbidly obese patients often elect for Roux-en-Y gastric bypass (RYGB), a form of bariatric surgery that triggers a remarkable 30% reduction in excess body weight and reversal of insulin resistance for those who are type II diabetic. A more complete understanding of the underlying molecular mechanisms that drive the complex metabolic reprogramming post-RYGB could lead to innovative non-invasive therapeutics that mimic the beneficial effects of the surgery, namely weight loss, achievement of glycemic control, or reversal of non-alcoholic steatohepatitis (NASH). To facilitate these discoveries, we hereby demonstrate the first multi-omic interrogation of a rodent RYGB model to reveal tissue-specific pathway modules implicated in the control of body weight regulation and energy homeostasis. In this study, we focus on and evaluate liver metabolism three months following RYGB in rats using both SWATH proteomics, a burgeoning label free approach using high resolution mass spectrometry to quantify protein levels in biological samples, as well as MRM metabolomics. The SWATH analysis enabled the quantification of 1378 proteins in liver tissue extracts, of which we report the significant down-regulation of Thrsp and Acot13 in RYGB as put...Continue Reading

References

Dec 16, 1997·Proceedings of the National Academy of Sciences of the United States of America·D E AgafonovA S Spirin
Dec 8, 1998·Journal of Gastrointestinal Surgery : Official Journal of the Society for Surgery of the Alimentary Tract·R E BrolinR P Cody
Dec 11, 1999·Nucleic Acids Research·M Kanehisa, S Goto
Jan 26, 2002·Pediatric Research·Sevasti B KoukouritakiRonald N Hines
Jul 20, 2002·Molecular Genetics and Metabolism·Denise L M GohMichael T Geraghty
Jul 15, 2003·BMC Medical Genetics·Veeriah SelvarajuArun Kumar
Oct 28, 2003·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Stefania RecalcatiGaetano Cairo
Dec 6, 2003·American Journal of Physiology. Cell Physiology·Alexander PushkinIra Kurtz
Oct 16, 2004·The Journal of Clinical Endocrinology and Metabolism·Judith KornerSharon L Wardlaw
Aug 30, 2006·Genome Biology·Shelley Halpain, Leif Dehmelt
Sep 29, 2007·Journal of Human Genetics·Biagio DidonaAlessandro Terrinoni
Oct 8, 2008·European Journal of Clinical Investigation·A B CrujeirasJ A Martínez
Apr 10, 2009·Molecular and Cellular Endocrinology·Grant W AndersonCary N Mariash
Jun 25, 2009·The Journal of Clinical Endocrinology and Metabolism·Jussi PihlajamäkiMary Elizabeth Patti
Aug 5, 2009·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Craig D FisherNathan J Cherrington
Feb 24, 2010·The Journal of Biological Chemistry·Tomoko IshizukaOsamu Okazaki
May 1, 2010·The Journal of Biological Chemistry·Ryosuke DoiNorio Ishida
May 26, 2010·European Journal of Clinical Investigation·José Manuel Fernández-RealWifredo Ricart
Sep 2, 2010·International Journal of Obesity : Journal of the International Association for the Study of Obesity·A C ShinH-R Berthoud
May 28, 2011·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Jun ZhangJianwu Tang
Jun 10, 2011·Biochemical and Biophysical Research Communications·Atul Kumar Pandey, Santosh Kumar Kar
Jan 14, 2012·Genetics in Medicine : Official Journal of the American College of Medical Genetics·Sandesh C S NagamaniBrendan Lee
Feb 22, 2012·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Hye Won KangDavid E Cohen
Mar 2, 2012·Journal of Nutrigenetics and Nutrigenomics·Isabel Bondia-PonsJ Alfredo Martínez
Apr 3, 2012·Biochimica Et Biophysica Acta·Mary C HuntStefan E H Alexson
Sep 22, 2012·The Journal of Clinical Investigation·J Scott GabrielsenDonald A McClain
Jan 22, 2013·Obesity Reviews : an Official Journal of the International Association for the Study of Obesity·E W ChanI C K Wong
Jan 23, 2013·Journal of Proteome Research·Florian SeyfriedElaine Holmes

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Citations

Oct 30, 2019·Journal of Proteome Research·Naoaki ArimaMasanori Aikawa

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