Multi-omics Analyses Reveal Synergistic Carbohydrate Metabolism in Streptococcus mutans-Candida albicans Mixed-Species Biofilms

Infection and Immunity
K EllepolaC J Seneviratne

Abstract

Candida albicans, a major opportunistic fungal pathogen, is frequently found together with Streptococcus mutans in dental biofilms associated with severe childhood caries (tooth decay), a prevalent pediatric oral disease. However, the impact of this cross-kingdom relationship on C. albicans remains largely uncharacterized. Here, we employed a novel quantitative proteomics approach in conjunction with transcriptomic profiling to unravel molecular pathways of C. albicans when cocultured with S. mutans in mixed biofilms. RNA sequencing and iTRAQ (isobaric tags for relative and absolute quantitation)-based quantitative proteomics revealed that C. albicans genes and proteins associated with carbohydrate metabolism were significantly enhanced, including sugar transport, aerobic respiration, pyruvate breakdown, and the glyoxylate cycle. Other C. albicans genes and proteins directly and indirectly related to cell morphogenesis and cell wall components such as mannan and glucan were also upregulated, indicating enhanced fungal activity in mixed-species biofilm. Further analyses revealed that S. mutans-derived exoenzyme glucosyltransferase B (GtfB), which binds to the fungal cell surface to promote coadhesion, can break down sucrose into...Continue Reading

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Citations

Jan 19, 2020·Applied and Environmental Microbiology·Lauren M O'ConnellVincent P Richards
Feb 13, 2020·Frontiers in Microbiology·Shane Thomas O'DonnellCatherine Stanton
Oct 9, 2020·Critical Reviews in Microbiology·Chaminda J SeneviratneGordon Ramage
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Nov 14, 2020·Scientific Reports·Joshua T Huffines, Jessica A Scoffield
Jun 3, 2020·Current Opinion in Microbiology·Andrea Hodgins-Davis, Teresa R O'Meara
May 19, 2021·Critical Reviews in Microbiology·Fazlurrahman KhanYoung-Mog Kim
May 22, 2021·Journal of Dental Research·N AlkharsJ Xiao
Jun 3, 2021·Antibiotics·Anne Caroline Morais CaldeirãoDouglas Roberto Monteiro
Jul 9, 2021·Biofouling·Arella Cristina Muniz BritoLeopoldina de Fátima Dantas de Almeida
Sep 8, 2021·Frontiers in Cellular and Infection Microbiology·Xin LyuFeng Chen

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Datasets Mentioned

BETA
PXD010261

Methods Mentioned

BETA
phosphotransferase
RNA-Seq
RNA assay
PCR

Software Mentioned

ProteinPilot
cBot
Bingo
DESeq R package
Perl scripts
DESeq
TopHat
house
HTSeq
Bowtie

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