Multi-omics analysis identifies genes mediating the extension of cell walls in the Arabidopsis thaliana root elongation zone

Frontiers in Cell and Developmental Biology
Michael H WilsonT Charlie Hodgman

Abstract

Plant cell wall composition is important for regulating growth rates, especially in roots. However, neither analyses of cell wall composition nor transcriptomes on their own can comprehensively reveal which genes and processes are mediating growth and cell elongation rates. This study reveals the benefits of carrying out multiple analyses in combination. Sections of roots from five anatomically and functionally defined zones in Arabidopsis thaliana were prepared and divided into three biological replicates. We used glycan microarrays and antibodies to identify the major classes of glycans and glycoproteins present in the cell walls of these sections, and identified the expected decrease in pectin and increase in xylan from the meristematic zone (MS), through the rapid and late elongation zones (REZ, LEZ) to the maturation zone and the rest of the root, including the emerging lateral roots. Other compositional changes included extensin and xyloglucan levels peaking in the REZ and increasing levels of arabinogalactan-proteins (AGP) epitopes from the MS to the LEZ, which remained high through the subsequent mature zones. Immuno-staining using the same antibodies identified the tissue and (sub)cellular localization of many epitopes...Continue Reading

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Citations

Aug 22, 2015·International Journal of Molecular Sciences·Adam BarradaBenoît Menand
Oct 23, 2015·Trends in Plant Science·Viktoriya AvramovaGerrit T S Beemster
Jun 9, 2016·Stem Cells International·Ligia S B BoisserandOlivier Detante
Apr 4, 2017·The Plant Journal : for Cell and Molecular Biology·Marta BjornsonKatayoon Dehesh
Sep 20, 2018·The Plant Journal : for Cell and Molecular Biology·Chuanying Fang, Jie Luo
Feb 20, 2019·Genes·Ishan AjmeraChungui Lu
Feb 18, 2020·Journal of Experimental Botany·Elena SalviSabrina Sabatini
Sep 6, 2017·Frontiers in Plant Science·Luís F GoulaoSara Amâncio
Mar 3, 2018·Communicative & Integrative Biology·Wendy Cajero SánchezAdriana Garay-Arroyo

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Datasets Mentioned

BETA
E-MEXP-2912

Methods Mentioned

BETA
dissection
Assay

Software Mentioned

R
Bioconductor
Cell Wall Navigator
Limma
Excel
PlnTFDB
ImaGene
NeuronJ
ImageJ
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