Multi-photon excitation imaging of dynamic processes in living cells and tissues

Reviews of Physiology, Biochemistry and Pharmacology
Richard K P BenningerD W Piston

Abstract

Over the past decade, two-photon microscopy has successfully made the transition from the laser laboratory into a true biological research setting. This has been due in part to the recent development of turnkey ultrafast laser systems required for two-photon microscopy, allowing ease of use in nonspecialist laboratories. The advantages of two-photon microscopy over conventional optical sectioning techniques are for greater imaging depths and reduced overall phototoxicity, as such enabling noninvasive intra-vital imaging of cellular and subcellular processes. Greater understanding of these advantages has allowed this technique to be more effectively utilized in a biological research setting. This review will cover the recent widespread uses of two-photon microscopy and highlight the wide range of physiological studies enabled in fields such as neurosciences, developmental biology, immunology, cancer biology, and endocrinology.

Citations

Mar 9, 2010·Chemical Reviews·Renatus W SinkeldamYitzhak Tor
Dec 11, 2008·American Journal of Physiology. Endocrinology and Metabolism·Subhadra C GunawardanaDavid W Piston
Oct 11, 2008·The Journal of Clinical Investigation·Lara R NymanAlvin C Powers
Jun 9, 2016·Journal of Cell Science·Ge LiMingming Hao
Apr 14, 2010·Journal of Microscopy·B-G WangK-J Halbhuber
Mar 12, 2010·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Yan SunJiapei Dai
Oct 20, 2010·Immunology and Cell Biology·Heather J MelicharEllen A Robey
Jan 26, 2012·Current Opinion in Urology·Samarpit RaiAshutosh Tewari
Apr 4, 2012·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Hellen C Ishikawa-AnkerholdGregor P C Drummen
May 28, 2013·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Yi Zhang, Jian Yang
Jul 1, 2016·Small·Xiaoding LouBen Zhong Tang
Apr 10, 2016·Neuroscience Bulletin·Zehua Li, Jiapei Dai
Dec 15, 2010·Photochemistry and Photobiology·Yu-Jer HwangJulia G Lyubovitsky
Jul 29, 2011·Journal of Gastrointestinal Surgery : Official Journal of the Society for Surgery of the Alimentary Tract·Yuhki MorimotoMasato Kusunoki
Jun 4, 2013·Current Protocols in Cell Biology·Richard K P Benninger, David W Piston
Nov 11, 2008·Neuron·Jeff W Lichtman, Stephen J Smith

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Current Opinion in Neurobiology
Yicong WuHari Shroff
Histochemistry and Cell Biology
Roberto WeigertAndrius Masedunskas
IEEE Journal of Selected Topics in Quantum Electronics : a Publication of the IEEE Lasers and Electro-optics Society
Claudio VinegoniRalph Weissleder
© 2022 Meta ULC. All rights reserved