Multi-Stacked Supported Lipid Bilayer Micropatterning through Polymer Stencil Lift-Off

Membranes
Yujie ZhuJose M Moran-Mirabal

Abstract

Complex multi-lamellar structures play a critical role in biological systems, where they are present as lamellar bodies, and as part of biological assemblies that control energy transduction processes. Multi-lamellar lipid layers not only provide interesting systems for fundamental research on membrane structure and bilayer-associated polypeptides, but can also serve as components in bioinspired materials or devices. Although the ability to pattern stacked lipid bilayers at the micron scale is of importance for these purposes, limited work has been done in developing such patterning techniques. Here, we present a simple and direct approach to pattern stacked supported lipid bilayers (SLBs) using polymer stencil lift-off and the electrostatic interactions between cationic and anionic lipids. Both homogeneous and phase-segregated stacked SLB patterns were produced, demonstrating that the stacked lipid bilayers retain lateral diffusivity. We demonstrate patterned SLB stacks of up to four bilayers, where fluorescence resonance energy transfer (FRET) and quenching was used to probe the interactions between lipid bilayers. Furthermore, the study of lipid phase behaviour showed that gel phase domains align between adjacent layers. The...Continue Reading

References

Jan 30, 2004·Biophysical Journal·Yoshihisa Kaizuka, Jay T Groves
May 21, 2005·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Nanjun ChuJohn F Nagle
Feb 1, 2007·Analytical Chemistry·Jose M Moran-MirabalDavid M Lin
Feb 25, 2009·Journal of Structural Biology·David H MurrayVolker Kiessling
Jun 30, 2009·Journal of Structural Biology·Minsub ChungSteven G Boxer
Dec 22, 2009·Integrative Biology : Quantitative Biosciences From Nano to Macro·Christine P TanClaudia Fischbach
Mar 2, 2010·Nature Nanotechnology·Steven LenhertHarald Fuchs
Feb 7, 2012·Small·Omkar A NafdaySteven Lenhert
Oct 23, 2012·Nature Materials·Lobat TayebiAtul N Parikh

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Citations

Oct 5, 2016·Langmuir : the ACS Journal of Surfaces and Colloids·Yujie Zhu, Jose Moran-Mirabal
Apr 12, 2017·The Journal of Physical Chemistry. B·Jonathan D WestAdam P Hitchcock
Dec 23, 2016·Journal of Biomedical Optics·Vahideh Farzam RadLobat Tayebi
Nov 1, 2018·Experimental Biology and Medicine·Sarina ChandBarbara Ciani
Jan 1, 2018·Advanced Functional Materials·Minjee KangCecilia Leal

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Methods Mentioned

BETA
fluorescence microscopy
fluorescence resonance
FRET
fluorescence recovery after photobleaching

Software Mentioned

NIS Elements

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