Multi-state, 4-aminopyridine-sensitive ion channels in human spermatozoa

Developmental Biology
Y GuS J Publicover

Abstract

Although ion channels are known to be pivotal to sperm function, the technical difficulty of applying electrophysiological techniques to spermatozoa has prevented significant progress in this area. This is due to the cell size and angular shape in combination with their motility. Using a refined technique, specifically for patch clamping spermatozoa, we have made recordings from human cells. This technique permitted approaches which enable functional analysis of sperm ion channels, including acquisition of inside-out patches, generation of averaged currents, and observation of the effects of pharmacological manipulation during prolonged recordings. As well as a low conductance (7 pS) channel and a 25-pS channel, the most striking finding was the presence of very high conductance, 4-aminopyridine-sensitive multistate channels resembling the non-selective cation channel of sea urchin and mouse spermatozoa. Application of 2 mM 4-aminopyridine (a dose sufficient to cause channel blockade) caused an instant and dramatic transition of motility in the sperm population increasing hyperactivated motility by more than 10-fold as assessed by computer-assisted semen analysis. Combined application of patch clamp and pharmacological investig...Continue Reading

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Citations

Mar 7, 2014·Pflügers Archiv : European journal of physiology·Alberto Darszon, Arturo Hernández-Cruz
Jun 23, 2009·Reproduction : the Official Journal of the Society for the Study of Fertility·Sarah CostelloStephen Publicover
Jun 7, 2011·Molecular Human Reproduction·Yuriy Kirichok, Polina V Lishko
Jan 25, 2013·The Journal of Biological Chemistry·Wardah AlasmariChristopher L R Barratt
Feb 15, 2013·Human Reproduction·Wardah AlasmariSenga K Oxenham
Feb 27, 2009·Cell Motility and the Cytoskeleton·D J SmithJ C Kirkman-Brown
Oct 28, 2011·Molecular Reproduction and Development·Mariano G BuffoneGeorge L Gerton
Jan 18, 2006·Molecular and Cellular Endocrinology·Timothy A QuillDavid L Garbers
Nov 26, 2009·Channels·Qiong-Yao TangChristopher J Lingle
Dec 14, 2005·Developmental Biology·Juan José AcevedoAlberto Darszon
Jun 13, 2015·Royal Society Open Science·E H OoiJ Kirkman-Brown
Jan 28, 2005·Biology of Reproduction·J P BarfieldT G Cooper
Jun 22, 2010·The Analyst·Celeste A MorrisLane A Baker
Jan 20, 2006·Molecular Human Reproduction·J P BarfieldT G Cooper
Dec 5, 2008·Systems Biology in Reproductive Medicine·Marcelo MarconiFernando Romero
Oct 31, 2019·Human Reproduction Update·Sean G BrownSarah J Martins da Silva
Jul 23, 2005·The Journal of Biological Chemistry·Anne E CarlsonDonner F Babcock
Mar 11, 2011·Biology of Reproduction·Haixin Chang, Susan S Suarez

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