Multi-walled carbon nanotube length as a critical determinant of bioreactivity with primary human pulmonary alveolar cells

Carbon
Sinbad SweeneyTeresa D Tetley

Abstract

Multiwalled carbon nanotube (MWCNT) length is suggested to critically determine their pulmonary toxicity. This stems from in vitro and in vivo rodent studies and in vitro human studies using cell lines (typically cancerous). There is little data using primary human lung cells. We addressed this knowledge gap, using highly relevant, primary human alveolar cell models exposed to precisely synthesized and thoroughly characterized MWCNTs. In this work, transformed human alveolar type-I-like epithelial cells (TT1), primary human alveolar type-II epithelial cells (ATII) and alveolar macrophages (AM) were treated with increasing concentrations of MWCNTs before measuring cytotoxicity, inflammatory mediator release and MAP kinase signalling. Strikingly, we observed that short MWCNTs (~0.6 µm in length) induced significantly greater responses from the epithelial cells, whilst AM were particularly susceptible to long MWCNTs (~20 µm). These differences in the pattern of mediator release were associated with alternative profiles of JNK, p38 and ERK1/2 MAP kinase signal transduction within each cell type. This study, using highly relevant target human alveolar cells and well defined and characterized MWCNTs, shows marked cellular responses t...Continue Reading

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Citations

Mar 2, 2016·Particle and Fibre Toxicology·Giulia ViettiSybille van den Brule
Oct 19, 2016·Journal of Controlled Release : Official Journal of the Controlled Release Society·Pedro M CostaKhuloud T Al-Jamal
Feb 23, 2020·Environmental Science and Pollution Research International·Mansour Rezazadeh Azari, Yousef Mohammadian
Jan 6, 2021·Journal of Applied Toxicology : JAT·Nhuan Thi DoSe-Hwa Kim
Aug 3, 2021·Oxidative Medicine and Cellular Longevity·Nemi MalhotraChung-Der Hsiao

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