Multianalyte detection using a capillary-based flow immunosensor

Analytical Biochemistry
U NarangF S Ligler

Abstract

A highly sensitive, dual-analyte detection system using capillary-based immunosensors has been designed for explosive detection. This model system consists of two capillaries, one coated with antibodies specific for 2,4,6-trinitrotoluene (TNT) and the other specific for hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) combined into a single device. The fused silica capillaries are prepared by coating anti-TNT and anti-RDX antibodies onto the silanized inner walls using a hetero-bifunctional crosslinker. After immobilization, the antibodies are saturated with a suitable fluorophorelabeled antigen. A "T" connector is used to continuously flow the buffer solution through the individual capillaries. To perform the assay, an aliquot of TNT or RDX or a mixture of the two analytes is injected into the continuous flow stream. In each capillary, the target analyte displaces the fluorophore-labeled antigen from the binding pocket of the antibody. The labeled antigen displaced from either capillary is detected downstream using two portable spectrofluorometers. The limits of detection for TNT and RDX in the multi-analyte formate are 44 fmol (100 microliters of 0.1 ng/ml TNT solution) and 224 fmol (100 microliters of 0.5 ng/ml RDX solution), r...Continue Reading

References

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Citations

Mar 27, 2001·Nature Reviews. Genetics·L Liotta, E Petricoin
Apr 4, 2006·Journal of Immunological Methods·J W ChungJ C Pyun
Nov 5, 2002·Analytical Biochemistry·Tiffanee M GreenGeorge P Anderson
Jul 1, 2008·Analytical and Bioanalytical Chemistry·Melissa S Meaney, Victoria L McGuffin
Jul 28, 2004·Analytical and Bioanalytical Chemistry·Michael G Weller
Sep 26, 2007·The Analyst·Callum J McHughDuncan Graham
Apr 23, 2008·The Analyst·Richard G SmithStephen Nicklin
Mar 19, 2008·Analytical and Bioanalytical Chemistry·Goran Klenkar, Bo Liedberg
Nov 13, 2008·Analytical and Bioanalytical Chemistry·Aristidis E NiotisSotirios E Kakabakos
Dec 25, 2002·Analytical Chemistry·Christos MastichiadisKonstantinos Misiako
Jun 13, 2001·Analytical Biochemistry·F SzurdokiD R Walt
Oct 27, 2001·Biosensors & Bioelectronics·S H Sheikh, A Mulchandani
Sep 1, 2005·Analytical Chemistry·Maxim Y BalakirevFrançois Chatelain
Oct 12, 1999·Analytical Chemistry·T E PlowmanW M Reichert
Feb 9, 1999·Analytical Chemistry·C A RoweF S Ligler
Apr 2, 2002·Analytical Chemistry·Kim E SapsfordFrances S Ligler

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