MultiBac: from protein complex structures to synthetic viral nanosystems.

BMC Biology
Martin PelosseImre Berger

Abstract

The MultiBac baculovirus/insect cell expression vector system was conceived as a user-friendly, modular tool-kit for producing multiprotein complexes for structural biology applications. MultiBac has allowed the structure and function of many molecular machines to be elucidated, including previously inaccessible high-value drug targets. More recently, MultiBac developments have shifted to customized baculoviral genomes that are tailored for a range of applications, including synthesizing artificial proteins by genetic code expansion. We review some of these developments, including the ongoing rewiring of the MultiBac system for mammalian applications, notably CRISPR/Cas9-mediated gene editing.

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Citations

Aug 29, 2018·Applied Microbiology and Biotechnology·Zhanqi DongMin-Hui Pan
Nov 10, 2018·Frontiers in Microbiology·Lena SchleicherJulia Steuber
Nov 11, 2019·Emerging Topics in Life Sciences·Imre BergerKapil Gupta

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Methods Mentioned

BETA
X-ray
glycosylation
transfection

Software Mentioned

biGBac
MultiBacMam
SynBac
MultiBac
MultiBacTAG

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