Multicenter comparison of three commercial methods for quantification of human immunodeficiency virus type 1 RNA in plasma.

Journal of Clinical Microbiology
R SchuurmanC Loveday

Abstract

Three procedures for the quantification of human immunodeficiency virus type 1 (HIV-1) RNA from plasma were compared at three laboratories. The comparison involved the Quantiplex branched DNA assay (version 1.0) by Chiron Diagnostics, the NASBA-QT assay by Organon Teknika, and the Amplicor Monitor assay by Roche Molecular Systems. The laboratories performed each of the three assays with the same sets of reconstructed HIV-1-infected human plasma samples, cross-sectionally collected clinical plasma samples and longitudinally collected plasma samples from patients starting zidovudine therapy. Analysis of the reconstruction panel results for interlaboratory variation demonstrated that no laboratory differences in results were detected for any of the assays. A comparison of the reproducibilities of duplicate samples analyzed by batch and in separate assay runs demonstrated that the reproducibilities of the test results were similar within one assay and appeared to be independent of the HIV-1 concentration. The best reproducibility was obtained with the Quantiplex assay, but all three assays demonstrated equal reliability, which was independent of batched or unbatched analysis of replicate samples. Differences in the absolute concent...Continue Reading

References

Jun 1, 1992·The Journal of Infectious Diseases·D T ScaddenJ E Groopman
Apr 15, 1995·Annals of Internal Medicine·J W MellorsP Gupta

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Citations

Feb 28, 2002·AIDS Research and Human Retroviruses·Rob SchuurmanUNKNOWN ENVA Working Group
Jul 10, 2004·AIDS Research and Human Retroviruses·Cheryl-Ann MonteiroCraig J Hoesley
Jul 23, 2009·AIDS Research and Human Retroviruses·Julio CollazosUNKNOWN Grupo Español para el Estudio Multifactorial de la Adherencia (Geema)
Jun 5, 2002·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Azra C GhaniFrank De Wolf
Nov 16, 2006·HIV Medicine·J CollazosUNKNOWN Grupo Español para el Estudio Multifactorial de la Adherencia (GEEMA)
Feb 5, 2005·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Erica Spackman, David L Suarez
Apr 20, 1999·AIDS·UNKNOWN Delta Coordinating Committee, UNKNOWN Delta Virology Committee
Jun 24, 2000·Journal of Acquired Immune Deficiency Syndromes : JAIDS·J MaselA L Lloyd
Jan 5, 2001·Journal of Acquired Immune Deficiency Syndromes : JAIDS·H GuevaraD Katzenstein
Nov 28, 2009·Epidemiology and Infection·J CollazosUNKNOWN Grupo Español para el Estudio Multifactorial de la Adherencia (GEEMA)
Dec 28, 1999·Proceedings of the National Academy of Sciences of the United States of America·N M FergusonR M Anderson
May 21, 1999·Analytical Biochemistry·K Wei, M J Stapleton
Jul 1, 1998·Clinical and Diagnostic Virology·R L Hodinka
Apr 4, 2000·Journal of Clinical Microbiology·R N ShepardS A Fiscus
Aug 30, 2001·Journal of Clinical Microbiology·K E Van VlietA M Van Loon
Dec 12, 2001·Archives of Pathology & Laboratory Medicine·D P FrancisW O Schalla
Oct 28, 1999·Archives of Pathology & Laboratory Medicine·F S Nolte
Apr 10, 2003·Archives of Pathology & Laboratory Medicine·Louise K HofherrWilliam O Schalla
Jan 10, 2002·Infectious Disease Clinics of North America·D WolkR Patel
Aug 16, 2005·Clinica Chimica Acta; International Journal of Clinical Chemistry·Rana Domiati-Saad, Richard H Scheuermann

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