Multicenter evaluation of PCR methods for detecting CMV DNA in blood donors

Transfusion
John D RobackMichael P Busch

Abstract

CMV DNA screening may be a useful adjunct to serologic tests in distinguishing potentially infectious blood donations from those that are "CMV-safe." However, there is currently no consensus on the optimal assay method for accurate detection of CMV DNA in donors. A blinded multicenter evaluation of seven CMV PCR assays was performed by five laboratories by using coded sets of analytical controls and donor blood samples. Five assays displayed sufficient sensitivity for donor screening, as judged by consistent detection of a minimum of 25 CMV genome equivalents (geq) in analytical controls constructed to contain from 1 to 100 CMV geq in background DNA from 250,000 cells, while the other two assays displayed inadequate sensitivity. Three sensitive assays, two based on nested PCR directed at the UL93 and UL32 regions of the CMV genome and another test (Monitor Assay, Roche), did not detect CMV DNA in samples from any of 20 pedigreed CMV-seronegative, Western blot-negative (S-/WB-) donors. Two other assays based on nested PCR occasionally detected CMV DNA in S-WB- samples, and one sensitive nested PCR assay directed at UL123 detected CMV DNA in a large proportion (85%) of S-WB- samples. Seven CMV PCR assays currently used for resear...Continue Reading

References

May 1, 1979·American Journal of Diseases of Children·R A BallardP A Riedel
Sep 1, 1991·The Journal of General Virology·J Taylor-WiedemanJ H Sinclair
Oct 7, 1989·BMJ : British Medical Journal·P StanierA S Tyms
Feb 1, 1981·The Journal of Pediatrics·A S YeagerC G Prober
Mar 1, 1983·Pediatric Infectious Disease·S P AdlerJ Baggett
Dec 6, 1994·Proceedings of the National Academy of Sciences of the United States of America·K KondoE S Mocarski
Aug 26, 1998·Transfusion·S J LeeM Johannesson
May 11, 1999·Journal of Virology·B Slobedman, E S Mocarski
Aug 7, 1999·The Journal of Infectious Diseases·F ZanghelliniR F Pass
Sep 30, 1999·Transfusion·W H Dzik, A Okayama

❮ Previous
Next ❯

Citations

Jul 19, 2002·Reviews in Medical Virology·John D Roback
Mar 6, 2004·The Journal of Infection·L C SpanoJ P G Leite
Oct 22, 2014·Nature Reviews. Urology·Fabrícia GimenesMarcia E L Consolaro
Oct 10, 2009·The American Journal of Pathology·Chukwuma OnyeagochaAndrew T Gewirtz
Dec 23, 2015·Pediatric Transplantation·Sanhita GangopadhyayKiran Upadhyay
Oct 24, 2007·American Journal of Clinical Pathology·Jeffrey S RossGraham Brock
May 23, 2008·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Sonali K SanghaviCharles R Rinaldo
Jan 1, 2008·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·John Sinclair
Dec 19, 2006·Transfusion Medicine Reviews·John D RobackChristopher D Hillyer
Apr 5, 2003·Transfusion·John D RobackMichael P Busch
Sep 26, 2003·Transfusion·Barbara GlockWolfgang R Mayr
Oct 29, 2013·Virulence·Tomasz JarzembowskiAlicja Dębska-Ślizień
Oct 25, 2016·Virology Journal·Maria-Cristina ArcangelettiAdriana Calderaro
Oct 16, 2004·Expert Review of Anti-infective Therapy·Audrey Schuetz, John D Roback
Jan 26, 2021·Frontiers in Cellular and Infection Microbiology·Biana BernshteinMichal Schwartz
Apr 30, 2021·Frontiers in Immunology·Sarah E JacksonMark R Wills

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.