Multicenter Evaluation of the Bruker MALDI Biotyper CA System for the Identification of Clinically Important Bacteria and Yeasts

American Journal of Clinical Pathology
Deborah A WilsonGary W Procop

Abstract

A report on the multicenter evaluation of the Bruker MALDI Biotyper CA System (MBT-CA; Bruker Daltonics, Billerica, MA) for the identification of clinically important bacteria and yeasts. In total, 4,399 isolates of medically important bacteria and yeasts were assessed in the MBT-CA. These included 2,262 aerobic gram-positive (AGP) bacteria, 792 aerobic gram-negative (AGN) bacteria 530 anaerobic (AnA) bacteria, and 815 yeasts (YSTs). Three processing methods were assesed. Overall, 98.4% (4,329/4,399) of all bacterial and yeast isolates were correctly identified to the genus and species/species complex level, and 95.7% of isolates were identified with a high degree of confidence. The percentage correctly identified and the percentage identified correctly with a high level of confidence, respectively, were as follows: AGP bacteria (98.6%/96.5%), AGN bacteria (98.5%/96.8%), AnA bacteria (98.5%/97.4%), and YSTs (97.8%/87.6%). The extended direct transfer method was only minimally superior to the direct transfer method for bacteria (89.9% vs 86.8%, respectively) but significantly superior for yeast isolates (74.0% vs 48.9%, respectively). The Bruker MALDI Biotyper CA System accurately identifies most clinically important bacteria an...Continue Reading

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Citations

Dec 10, 2019·Acta Microbiologica Et Immunologica Hungarica·Nurver Ulger ToprakUNKNOWN ESCMID Study Group for Anaerobic Infections (ESGAI)**
Jan 15, 2020·The Protein Journal·Anna S PavlovaAirat R Kayumov
Jan 4, 2019·Current Microbiology·Israa Mohamad El-NemrMohammad Rubayet Hasan
Jan 7, 2020·Mass Spectrometry Reviews·Jia-Jia CuiJi-Ye Yin

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