PMID: 9431944Feb 12, 1998Paper

Multicenter evaluation of the Clostridium difficile TOX A/B TEST

Journal of Clinical Microbiology
D M LyerlyR Schwalbe

Abstract

Clostridium difficile, the primary cause of nosocomial diarrhea in the United States and many other industrialized countries, is recognized as a major health concern because of its ability to cause severe intestinal disease leading to complications such as relapses and infections due to vancomycin-resistant enterococci. The disease results from two toxins, toxins A and B, produced by this pathogen. In this study, we evaluated the TOX A/B TEST, a new 1-h enzyme immunoassay (EIA) that detects toxins A and B. We compared the test with the tissue culture assay, which is recognized as the "gold standard" for C. difficile testing. Evaluations were performed in-house at TechLab, Inc. (Blacksburg, Va.) and off-site at four clinical laboratories. Of 1,152 specimens tested, 165 were positive by the TOX A/B TEST and tissue culture and 973 were negative by both tests. The sensitivity and specificity were 92.2 and 100%, respectively. The positive and negative predictive values were 100 and 98.6%, respectively, and the correlation of the TOX A/B TEST with tissue culture was 98.8%. When discrepant samples were resolved by culture, the sensitivity and specificity were 93.2 and 98.9%, respectively. The positive and negative predictive values we...Continue Reading

References

Feb 1, 1979·Journal of Clinical Microbiology·W L GeorgeS M Finegold
Jan 1, 1992·Journal of Medical Microbiology·R D Martinez, T D Wilkins
May 1, 1992·Molecular & General Genetics : MGG·C von Eichel-StreiberM Sauerborn
Feb 1, 1990·Infection and Immunity·C H DoveJ L Johnson
Jul 11, 1990·Nucleic Acids Research·L A BarrosoT D Wilkins
Jul 5, 1986·Lancet·B S BenderJ G Bartlett
Jan 26, 1989·The New England Journal of Medicine·L V McFarlandW E Stamm
Jan 1, 1988·European Journal of Pediatrics·M DelméeG Francois
Aug 1, 1987·Gastroenterology·G TriadafilopoulosJ T LaMont
Feb 1, 1985·Infection and Immunity·D M LyerlyT D Wilkins
Jul 1, 1995·Molecular Microbiology·C von Eichel-StreiberS Sartingen
Jun 1, 1993·Journal of Medical Microbiology·C DepitreG Corthier
Jan 1, 1996·Journal of Medical Microbiology·G A GreenH Monteil

❮ Previous
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Citations

Jul 25, 2000·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·J S WeeseJ F Prescott
Oct 6, 2000·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·J S WeeseJ F Prescott
May 30, 2002·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Karen W PostJ Glenn Songer
Jun 23, 2004·QJM : Monthly Journal of the Association of Physicians·K A O'ConnorD O'Mahony
May 5, 2006·Veterinary Pathology·M K Keel, J G Songer
Nov 2, 2006·Digestive Diseases and Sciences·Jacob YahavHaim Shmuely
Dec 25, 2007·The Korean journal of laboratory medicine·Soo Jin YooBo Moon Shin
Dec 20, 2008·American Journal of Clinical Pathology·Rosemary C SheCathy A Petti
Oct 16, 2008·The American Journal of Gastroenterology·M Raffat JaberMark E Reeves
Jul 18, 2000·Transactions of the Royal Society of Tropical Medicine and Hygiene·I ZuluM J Farthing
Mar 29, 2005·Comprehensive Therapy·Jonathan Gonenne, Darrell S Pardi
Apr 12, 2003·Journal of the American Veterinary Medical Association·K Gary MagdesianJohn E Madigan
Nov 9, 2007·Digestive Diseases and Sciences·Zmira SamraJihad Bishara
Dec 9, 2008·Current Opinion in Organ Transplantation·David J Riddle, Erik R Dubberke
May 5, 2009·The Korean journal of laboratory medicine·Bo-Moon ShinHye Jun Oh
Dec 3, 2016·Journal of Veterinary Internal Medicine·L G ArroyoJ S Weese
Aug 1, 2002·Current Gastroenterology Reports·Munshi MoyenuddinChristopher A Ohl
Dec 25, 2007·The Korean journal of laboratory medicine·Bo Moon Shin, Eun Young Kuak
Oct 7, 2006·Current Opinion in Gastroenterology·L C Ee, M B Cohen
Oct 29, 2009·Kansenshōgaku zasshi. The Journal of the Japanese Association for Infectious Diseases·Hideko SugiuraKyoko Takeda
May 10, 2001·Journal of the American Veterinary Medical Association·J S WeeseH R Staempfli
Sep 12, 2000·Current Gastroenterology Reports·C S Alcantara, R L Guerrant
Jan 11, 2007·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·H RüssmannR Schaumann
Dec 9, 2003·Journal of Veterinary Internal Medicine·J S Weese, J Armstrong
Jan 6, 2021·Journal of Clinical Medicine·Andrew M SkinnerStuart Johnson
Aug 2, 2006·Gastroenterology Clinics of North America·Saima Aslam, Daniel M Musher
Jun 23, 1999·The American Journal of Medicine·J Aranda-Michel, R A Giannella
Apr 15, 2000·Diagnostic Microbiology and Infectious Disease·W E AldeenK C Carroll
Apr 4, 2000·Journal of Clinical Microbiology·A P LimayeT R Fritsche
Apr 19, 2003·Journal of Clinical Microbiology·Stuart JohnsonDale N Gerding
Jul 17, 1998·Journal of Clinical Microbiology·H KatoE B Wasito
Jul 25, 2009·Journal of Clinical Microbiology·Bo-Moon ShinJ Glenn Songer

❮ Previous
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