Multicenter evaluation of the flow cytometry T-cell crossmatch: results from the American Society of Histocompatibility and Immunogenetics-College of American Pathologists proficiency testing program

Transplantation
J C ScornikJ W Langley

Abstract

The performance characteristics and interlaboratory comparisons of the T-cell flow cytometry crossmatch remain largely unknown. This study was performed using data from the ASHI-CAP proficiency testing program. Four unknown sera and two unknown cells were sent to participating laboratories twice a year for 4 years. In one survey in which different crossmatch techniques were compared, flow cytometry was slightly more sensitive than the antiglobulin method and considerably more sensitive than direct cytotoxicity. However, the proportion of participants in any given survey detecting antibodies in all sera expected to be positive was 50-60% and has not changed over the years. Failure to detect antibodies correlated with low antibody concentration, diluting the unknown serum by the testing laboratory, and with the instrument used. False positive results with normal sera were infrequent. Fluorescence intensity values were not standardized and were highly variable, but when fluorescence units reported by individual laboratories were divided by their own positive-negative cutoff values, results from different centers were more comparable. In general, fluorescence-to-cutoff ratios >5 correlated with complement binding activity, whereas ...Continue Reading

Citations

May 16, 2002·Transplantation Proceedings·K MichalskaA Górski
Sep 24, 1998·Transplantation Proceedings·R J Duquesnoy
Sep 24, 1998·Transplantation Proceedings·J M RebibouP Tiberghien
Sep 27, 2000·Transplant Immunology·J M RebibouP Tiberghien
Dec 29, 1998·Current Opinion in Nephrology and Hypertension·A W Harmer
Jan 8, 2005·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Martin S ZandNufatt Leong
Jan 6, 2007·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Barbara H McGovernTamsin A Knox
Mar 21, 2009·Transplantation Reviews·Kathryn Tinckam
May 24, 2008·Experimental and Molecular Pathology·Julio C Delgado, David D Eckels
Sep 4, 2004·Clinical Transplantation·J M RebibouP Tiberghien
Nov 18, 2003·Immunological Reviews·Maria P Hernandez-FuentesRobert I Lechler
Nov 25, 2003·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Howard M GebelPeter Nickerson
Sep 13, 2006·Néphrologie & thérapeutique·Eric Thervet, Christophe Legendre
Jan 10, 2018·World Journal of Transplantation·Mohammed Mahdi AlthafAhmed Mostafa Halawa
Mar 7, 2019·HLA : Immune Response Genetics·Daniel S RamonLeo Li-Ying Chan
Jul 4, 2015·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·Andrew M South, Paul C Grimm
Mar 24, 2000·Pediatric Transplantation·M P EmondsR Van Damme-Lombaerts
Feb 22, 2002·Journal of the American Society of Nephrology : JASN·Shamila MauiyyediRobert B Colvin

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