Multicenter evaluation of the LightCycler MRSA advanced test, the Xpert MRSA Assay, and MRSASelect directly plated culture with simulated workflow comparison for the detection of methicillin-resistant Staphylococcus aureus in nasal swabs

The Journal of Molecular Diagnostics : JMD
Rodney C ArcenasArundhati Rao

Abstract

Rapid detection of nasal colonization with methicillin-resistant Staphylococcus aureus (MRSA) followed by appropriate infection control procedures reduces MRSA infection and transmission. We compared the performance and workflow of two Food and Drug Administration-approved nucleic acid amplification assays, the LightCycler MRSA Advanced Test and the Xpert MRSA test, with those of directly plated culture (MRSASelect) using 1202 nasal swabs collected at three U.S. sites. The sensitivity of the LightCycler test (95.2%; 95% CI, 89.1% to 98.4%) and Xpert assay (99%; 95% CI, 94.8% to 100%) did not differ compared with that of culture; the specificity of the two assays was identical (95.5%; 95% CI, 94.1% to 96.7%) compared with culture. However, sequencing performed on 71 samples with discordant results among the three methods confirmed the presence of MRSA in 40% of samples that were positive by both molecular methods but negative by culture. Workflow analysis from all sites including batch runs revealed average hands-on sample preparation times of 1.40, 2.35, and 1.44 minutes per sample for the LightCycler, Xpert, and MRSASelect methods, respectively. Discrete event simulation analysis of workflow efficiencies revealed that the Ligh...Continue Reading

References

Jan 23, 2003·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Susan S Huang, Richard Platt
Apr 7, 2006·Journal of Clinical Microbiology·M W D WrenA P R Wilson
Jun 27, 2006·BMJ : British Medical Journal·David H WyllieTim E A Peto
Apr 13, 2007·Journal of Clinical Microbiology·Patrice FrancoisJacques Schrenzel
Oct 18, 2007·JAMA : the Journal of the American Medical Association·R Monina KlevensUNKNOWN Active Bacterial Core surveillance (ABCs) MRSA Investigators
Mar 19, 2008·Annals of Internal Medicine·Ari RobicsekLance R Peterson
Apr 25, 2008·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Catherine LiuHenry F Chambers
Jun 6, 2008·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Rupak Datta, Susan S Huang
Apr 25, 2009·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Christoph K Naber
Jul 23, 2009·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·Katherine HardyPeter M Hawkey
Dec 3, 2009·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·E Tacconelli
Jan 8, 2010·The New England Journal of Medicine·Lonneke G M BodeMargreet C Vos
Jan 15, 2010·Journal of Clinical Microbiology·Lance R Peterson, Daniel J Diekema
Feb 19, 2010·Journal of Clinical Microbiology·Surbhi Malhotra-KumarUNKNOWN MOSAR WP2 Study Team
Feb 27, 2010·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·Eilish CreamerHilary Humphreys
Oct 15, 2010·Journal of Clinical Microbiology·Surbhi Malhotra-KumarUNKNOWN MOSAR WP2 Study Team

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Citations

Jan 13, 2018·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·David B BanachTimothy L Wiemken

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