Multicenter Evaluation of the Xpert Carba-R Assay for Detection of Carbapenemase Genes in Gram-Negative Isolates

Journal of Clinical Microbiology
M TraczewskiCarba-R Study Team

Abstract

This multicenter study evaluated the performance of the Cepheid Xpert Carba-R assay, a qualitative PCR test designed for the rapid detection of blaKPC, blaNDM, blaVIM, blaIMP, and blaOXA-48 carbapenem resistance genes from bacterial isolates grown on blood agar or MacConkey agar. The results were compared to those obtained from bidirectional DNA sequence analysis of nucleic acid extracted from pure colonies. Isolates of Enterobacteriaceae, Pseudomonas aeruginosa, and Acinetobacter baumannii that tested as either intermediate or resistant to a carbapenem antibiotic were analyzed. A total of 467 isolates were evaluated, including prospectively collected clinical isolates, frozen isolates, and a group of contrived broth specimens sent by a central reference laboratory. The assay was run on the GeneXpert platform and took 48 min, with less than 1 min of hands-on time. Compared to the results of the reference methods, the overall sensitivity of the assay was 100% (95% confidence interval [CI], 99.0 to 100%) for isolates grown on both blood and MacConkey agars. Overall specificity was 98.1% (95% CI, 93.1 to 99.8%) and 97.1% (95% CI, 91.7 to 99.4%) for blood and MacConkey agars, respectively. This platform, previously demonstrated to ...Continue Reading

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Citations

Jul 18, 2019·Expert Review of Molecular Diagnostics·Michaela SimonJonathan Jantsch
Jul 17, 2019·Antimicrobial Agents and Chemotherapy·Honghui WangAnthony F Suffredini
Feb 8, 2020·Expert Review of Molecular Diagnostics·Peter J DaileyMark Holodniy
Mar 7, 2020·Future Science OA·Ann A Elshamy, Khaled M Aboshanab
Mar 28, 2020·Journal of Clinical Microbiology·Christopher F LoweGordon Ritchie
Feb 13, 2021·Enfermedades infecciosas y microbiología clínica·Alba de la Rica-MartínezAntonio Galiana-Cabrera
Feb 16, 2021·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·Alex M TrzebuckiMatthew S Simon
Mar 26, 2021·Transactions of the Royal Society of Tropical Medicine and Hygiene·Jaffar A Al-TawfiqAli M Bazzi
Apr 20, 2021·Journal of Microbiological Methods·Katja ProbstAlexander H Dalpke
Apr 20, 2021·Enfermedades infecciosas y microbiología clínica·Marina OviañoGermán Bou
May 1, 2021·Expert Review of Molecular Diagnostics·Michael R JacobsDaniel D Rhoads
Aug 6, 2021·Diagnostic Microbiology and Infectious Disease·Giuseppe RusselloEdoardo Carretto

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