Multicenter Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry Study for Identification of Clinically Relevant Nocardia spp

Journal of Clinical Microbiology
Sara J BlosserAdrian M Zelazny

Abstract

This multicenter study analyzed Nocardia spp., including extraction, spectral acquisition, Bruker matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) identification, and score interpretation, using three Nocardia libraries, the Bruker, National Institutes of Health (NIH), and The Ohio State University (OSU) libraries, and compared the results obtained by each center. A standardized study protocol, 150 Nocardia isolates, and NIH and OSU Nocardia MALDI-TOF MS libraries were distributed to three centers. Following standardized culture, extraction, and MALDI-TOF MS analysis, isolates were identified using score cutoffs of ≥2.0 for species/species complex-level identification and ≥1.8 for genus-level identification. Isolates yielding a score of <2.0 underwent a single repeat extraction and analysis. The overall score range for all centers was 1.3 to 2.7 (average, 2.2 ± 0.3), with common species generally producing higher average scores than less common ones. Score categorization and isolate identification demonstrated 86% agreement between centers; 118 of 150 isolates were correctly identified to the species/species complex level by all centers. Nine strains (6.0%) were not identified by any c...Continue Reading

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Citations

Nov 3, 2016·Diagnostic Microbiology and Infectious Disease·Victoria GirardGéraldine Durand
Dec 15, 2017·British Journal of Hospital Medicine·Conor BowmanStephanie Wilmore
Nov 10, 2017·Journal of Clinical Microbiology·Patricia S ConvilleAdrian M Zelazny
Jul 16, 2019·Expert Review of Molecular Diagnostics·Markus KostrzewaArthur B Pranada
Jul 4, 2020·Current Opinion in Organ Transplantation·Jennifer L Saullo, Rachel A Miller
Jun 27, 2018·Journal of Clinical Microbiology·Kirstie R HinsonSiu-Kei Chow
Jun 23, 2019·Current Infectious Disease Reports·Marion Hemmersbach-MillerJennifer L Saullo
Nov 7, 2017·Journal of Veterinary Internal Medicine·S Yaemsiri, J E Sykes
Aug 19, 2018·Biotechnology Letters·Hala S HelalHamdallah Zedan
Dec 31, 2020·Infection and Drug Resistance·Emmanuel LafontDavid Lebeaux
May 27, 2021·BMJ Case Reports·Claudio Tirso AcevedoSilvio Daniel Brugger
Jul 9, 2021·Case Reports in Infectious Diseases·Muhammad EffendiJeffrey E Topal
Aug 21, 2021·BMC Infectious Diseases·Chukwunyelu EnwezorNiyati Jakharia
May 19, 2020·Medical Principles and Practice : International Journal of the Kuwait University, Health Science Centre·Shalini Dewan Duggal, Tulsi Das Chugh

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