Multicenter study using paraffin-embedded tumor tissue testing PITX2 DNA methylation as a marker for outcome prediction in tamoxifen-treated, node-negative breast cancer patients

Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology
Nadia HarbeckRalf Lesche

Abstract

We recently reported DNA methylation of the paired-like homeodomain transcription factor 2 (PITX2) gene to be strongly correlated with increased risk of recurrence in node-negative, hormone receptor-positive, tamoxifen-treated breast cancer patients using fresh frozen specimens. Aims of the present study were to establish determination of PITX2 methylation for routine analysis in formalin-fixed paraffin-embedded (FFPE) breast cancer tissue and to test PITX2 DNA methylation as a biomarker for outcome prediction in an independent patient cohort. Real-time polymerase chain reaction (PCR) technology was validated for FFPE tissue by comparing methylation measurements in FFPE specimens with those in fresh frozen specimens from the same tumor. The impact of PITX2 methylation on time to distant metastasis was then evaluated in FFPE specimens from hormone receptor-positive, node-negative breast cancer patients (n = 399, adjuvant tamoxifen monotherapy). Reproducibility of the PCR assay in replicate measurements (r(s) > or = 0.95; n = 150) and concordant measurements between fresh frozen and FFPE tissues (r(s) = 0.81; n = 89) were demonstrated. In a multivariate model, PITX2 methylation added significant information (hazard ratio = 2.35; ...Continue Reading

References

Dec 4, 1998·British Journal of Cancer·N KromanM Melbye
Jun 18, 1999·The Journal of Clinical Endocrinology and Metabolism·I Pellegrini-BouillerA Enjalbert
Mar 24, 2000·Trends in Genetics : TIG·S B Baylin, J G Herman
Feb 24, 2001·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·A LebeauU Löhrs
Apr 21, 2001·Blood·M ToyotaJ P Issa
Feb 2, 2002·Nature·Laura J van 't VeerStephen H Friend
Feb 13, 2002·The American Journal of Pathology·Ulrich LehmannHans Kreipe
Apr 3, 2003·Nature Reviews. Cancer·Peter W Laird
Jun 5, 2003·Molecular Pathology : MP·S BoninG Stanta
Nov 6, 2003·International Journal of Cancer. Journal International Du Cancer·Mary Jo FacklerPedram Argani
Nov 25, 2003·The New England Journal of Medicine·James G Herman, Stephen B Baylin
Jul 17, 2004·Neoplasia : an International Journal for Oncology Research·Bernard W FutscherFrederick E Domann
Dec 14, 2004·The New England Journal of Medicine·Soonmyung PaikNorman Wolmark
Mar 10, 2005·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Laura J van't VeerDaniel F Hayes
Jul 28, 2006·Cold Spring Harbor Symposia on Quantitative Biology·J G Herman
Oct 5, 2006·Nature Clinical Practice. Oncology·Jan BogaertsUNKNOWN TRANSBIG consortium
Oct 31, 2006·Annals of Oncology : Official Journal of the European Society for Medical Oncology·A GoldhirschUNKNOWN St Gallen Expert Panel Members
Nov 10, 2006·Clinical Breast Cancer·Joseph A Sparano
May 5, 2007·Virchows Archiv : an International Journal of Pathology·Ji Shin Lee
Jul 3, 2007·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Sabine MaierUNKNOWN European Organisation for Research and Treatment of Cancer (EORTC) PathoBiology group
Aug 7, 2007·Annals of Oncology : Official Journal of the European Society for Medical Oncology·A GoldhirschUNKNOWN 10th St. Gallen conference

❮ Previous
Next ❯

Citations

Apr 9, 2009·Breast Cancer Research and Treatment·Evangelia-Ourania FourkalaMartin Widschwendter
Jun 26, 2013·Cancer Causes & Control : CCC·Chun ChaoMary Helen Black
Jan 27, 2010·Journal of Mammary Gland Biology and Neoplasia·Thushangi N PathirajaSteffi Oesterreich
May 15, 2013·Journal of Mammary Gland Biology and Neoplasia·Jacqueline M VeltmaatEsta Sterneck
Feb 26, 2009·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Meng Hua TaoJo L Freudenheim
Jun 11, 2009·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Michael T McCabePaula M Vertino
Nov 22, 2012·Breast Cancer Research : BCR·Warwick J Locke, Susan J Clark
Nov 4, 2009·Annals of Surgical Oncology·Craig A MessickMatthew F Kalady
Aug 13, 2010·Biomarkers in Medicine·Rudolf NapieralskiManfred Schmitt
Oct 27, 2009·Future Oncology·John Wm MartensNadia Harbeck
Oct 29, 2013·Clinical & Experimental Metastasis·I VelaE M Gardiner
Jun 19, 2014·Journal of Cancer Research and Clinical Oncology·Kirill LitovkinMathieu Bollen
Feb 28, 2013·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Jong-Lyel RohWayne M Koch
Sep 18, 2014·Genes·Thomas Mikeska, Jeffrey M Craig
Jun 19, 2012·Expert Review of Molecular Diagnostics·Thomas MikeskaAlexander Dobrovic
Jan 1, 2012·Drug Discovery Today. Disease Mechanisms·Kerrie B BoukerRobert Clarke
Oct 22, 2014·Breast Cancer Research : BCR·Jia-Min B PangStephen B Fox
Mar 23, 2010·The Journal of Molecular Diagnostics : JMD·Philipp SchatzArndt Hartmann
Nov 8, 2012·International Journal of Cancer. Journal International Du Cancer·Jia-Xing ZhangYuan Zhao
May 11, 2012·ANZ Journal of Surgery·Adam Frankel
Oct 24, 2014·Asian Pacific Journal of Cancer Prevention : APJCP·Wan Faiziah Wan Abdul RahmanHasnan Jaafar
Jun 27, 2015·Hormones & Cancer·Shweta R NayakSteffi Oesterreich
Sep 15, 2012·Epigenetics : Official Journal of the DNA Methylation Society·Jing ShenRegina M Santella
Jan 14, 2009·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·M J DuffyUNKNOWN EORTC PathoBiology Group
Aug 1, 2012·Biochimie·Alexandre How KitJörg Tost
May 29, 2016·Drug Discovery Today·Tommaso De MarchiJohn W M Martens
Nov 3, 2016·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Eva JezkovaJan Danko
Nov 9, 2016·Genomics·Maria Fernanda Setúbal Destro RodriguesFabio Daumas Nunes
May 23, 2015·PLoS Computational Biology·Matthew H UngChao Cheng
Jun 18, 2017·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Qiling LiQing Song
Jul 26, 2018·Biomarkers in Medicine·Paola Parrella
Apr 22, 2014·Biomedical Reports·Yuanyuan DongShulin Yang
Mar 5, 2020·Technology in Cancer Research & Treatment·Xiao-Ran LiuHui-Ping Li
Sep 20, 2017·The Cancer Journal·K Wyatt McMahonNita Ahuja

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