Multicentre evaluation of a real-time PCR assay to detect genes encoding clinically relevant carbapenemases in cultured bacteria

International Journal of Antimicrobial Agents
Matthew J EllingtonPeter Hawkey

Abstract

The performance and portability of a multiplex real-time PCR assay to detect KPC, NDM, OXA-48-like and VIM carbapenemase gene families from bacterial isolates was assessed using Rotor-Gene Q and ABI 7500 instruments. Gram-negative bacterial isolates (n=502) were comprised of 100 isolates each with KPC, NDM, VIM or OXA-48-like carbapenemases (including 17 with OXA-181) and 2 isolates with NDM+OXA-48-like enzymes (including 1 with OXA-181) as well as 100 assay-negative isolates comprised of 24 IMP-producers, 24 carbapenem-resistant isolates with no known carbapenemase gene and 52 extended-spectrum β-lactamase-producing carbapenem-susceptible isolates. A multicentre evaluation was carried out in five laboratories using a subset of 100 isolates comprised of 22 isolates each with KPC, NDM, OXA-48-like or VIM alleles and 12 isolates that were negative for the assay targets. Initial validation of the assay on both the Rotor-Gene Q and ABI 7500 instruments demonstrated 100% sensitivity amongst the 402 isolates that were positive for KPC, NDM, OXA-48-like (including OXA-181) and VIM carbapenemase genes, whilst the 100 assay-negative samples were correctly identified indicating 100% specificity. During the multicentre evaluation the same...Continue Reading

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Citations

Jan 14, 2017·Environmental Science and Pollution Research International·Jèssica SubiratsCarles M Borrego
Oct 25, 2017·Bulletin of Experimental Biology and Medicine·I S BalashovT V Priputnevich
Jun 20, 2018·Antimicrobial Agents and Chemotherapy·Anna VickersDavid M Livermore
Apr 5, 2017·Antimicrobial Agents and Chemotherapy·Anita CheruvankyAmy J Mathers
Nov 2, 2018·Journal of Clinical Microbiology·Shannon KatiyoGauri Godbole
Apr 30, 2019·The Journal of Antimicrobial Chemotherapy·Carolyne HornerUNKNOWN BSAC Resistance Surveillance Standing Committee
Dec 12, 2017·The Journal of Antimicrobial Chemotherapy·David M LivermoreNeil Woodford
Apr 11, 2019·European Journal of Microbiology & Immunology·Hans KollendaMohamed Ben Moussa
Sep 5, 2019·Frontiers in Microbiology·Xiaoyan CuiHong Du
Apr 15, 2018·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Amin Abdelfattah AqelMatthew J Ellington
Dec 28, 2018·The Journal of Antimicrobial Chemotherapy·Shazad MushtaqDavid M Livermore
Jun 13, 2017·The Journal of Antimicrobial Chemotherapy·Danièle MeunierKatie L Hopkins
Dec 12, 2020·The Journal of Antimicrobial Chemotherapy·Shazad MushtaqDavid M Livermore

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