Multicolor and Ultrasensitive Enzyme-Linked Immunosorbent Assay Based on the Fluorescence Hybrid Chain Reaction for Simultaneous Detection of Pathogens

Journal of Agricultural and Food Chemistry
Xi LvWeihua Lai

Abstract

Various pathogens may coexist in one sample; however, detection methods that rely on traditional selective culture media or immune agents designed specifically for a certain target are unsuitable for multiple targets. It is important to develop a simultaneous and sensitive detection method for multiple pathogens. Here, a multicolor and ultrasensitive enzyme-linked immunosorbent assay (ELISA) platform based on the fluorescence hybridization chain reaction (HCR) was developed. In the assay, multicolor fluorescence concatemers formed as signal amplifiers and signal reporters in the presence of target pathogens. When HCR occurred, Escherichia coli O157:H7, Salmonella serotype Choleraesuis, and Listeria monocytogenes were detected simultaneously with three different fluorescences. Additionally, the limits of detection for E. coli O157:H7, Salmonella Choleraesuis, and L. monocytogenes were 3.4 × 101, 6.4 × 100, and 7.0 × 101 CFU/mL, respectively. The assay achieved ultrasensitive, specific, and simultaneous detection of three pathogens and can be applied to the detection of pathogens in milk samples. Therefore, this multicolor and ultrasensitive ELISA platform has great potential in the application of simultaneous detection of pathog...Continue Reading

References

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Citations

Jul 31, 2020·Analytical Methods : Advancing Methods and Applications·Yuexin LiuXianghui Meng
Jun 4, 2020·Biosensors·Athmar A AliSalam A Ibrahim
Dec 19, 2020·Comprehensive Reviews in Food Science and Food Safety·Ruyuan ZhangZisheng Luo
Apr 27, 2021·Critical Reviews in Microbiology·Leonardo Lopes-LuzSamira Bührer-Sékula
May 4, 2021·Talanta·Elham SheikhzadehMohammed Zourob
May 20, 2021·Journal of Agricultural and Food Chemistry·Panting SangHe Qian

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