Multicolor Nitrogen-Doped Carbon Dots for Live Cell Imaging

Journal of Biomedical Nanotechnology
Fengyi DuQixiang Shao

Abstract

Doping carbon dots with nitrogen atoms considerably enhances their fluorescence properties. However, the mechanism by which the carbon dots are doped is not fully understood. We developed a facile bottom-up hydrothermal carbonization (HTC) process that uses glucose and glycine as precursors for the synthesis of photoluminescent nitrogen-doped carbon dots. The as-prepared nitrogen-doped carbon dots were mono-dispersed spherical particles with a diameter of -2.8 nm. The doped nitrogen atoms assumed pyridinic type and pyrrolic type configurations to participate in the nanocrystal structure of the carbon dots. It appeared that the nitrogen doping introduces a new internal structure. The aqueous solution of nitrogen-doped carbon dots showed excitation wavelength-dependent multicolor photoluminescence. Further, these nitrogen-doped carbon dots readily entered the cytoplasm of A549 cancer cells and showed no significant cytotoxicity. The internalized nitrogen-doped carbon dots were localized to the cell membrane and cytoplasm, particularly around the nucleus. Further, the as-prepared, biocompatible, nitrogen-doped carbon dots demonstrated the potential to be used as fluorescent probes for multicolor live cell labeling, tracking, and i...Continue Reading

Citations

Dec 24, 2016·ACS Applied Materials & Interfaces·Vuk UskokovićVictoria M Wu
Jan 21, 2016·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Ji FengJian-Hua Wang
Jul 14, 2019·Methods : a Companion to Methods in Enzymology·Shuang CongMingqian Tan
Jun 2, 2020·ACS Omega·Binesh UnnikrishnanHuan-Tsung Chang

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