PMID: 3747527Aug 1, 1986Paper

Multicomponent analysis of bile acids in faeces by anion exchange and capillary column gas-liquid chromatography: application in oxytetracycline treated subjects

Journal of Steroid Biochemistry
J T KorpelaH Adlercreutz

Abstract

A method is described for the multicomponent analysis of bile acids in faeces. Following homogenization and extraction, bile acids are separated into several groups according to their mode of conjugation by anion exchange chromatography on DEAE-Sephadex A-25 in the acetate form. The different conjugate groups are then processed separately and quantified using capillary column gas-liquid chromatography (GC), the identity of the individual bile acid components being established by gas chromatography-mass spectrometry (GC-MS). The method permits the quantitative determination, with good precision, of unconjugated bile acids, their saponifiable derivatives, and their glycine-, taurine- and sulfated conjugates. In five healthy men the mean daily excretion of bile acids was 472 +/- 21.8 mumol (SEM) and the mean faecal concentration 7.74 +/- 1.07 mumol (SEM)/g dry weight. Free bile acids constituted 74.8% +/- 5.8% (SEM), saponifiable conjugates 23.5% +/- 5.8% (SEM) glycine-conjugates 0.7% +/- 0.2% (SEM), taurine-conjugates 0.2% +/- 0.07% (SEM), and their sulfated conjugates 0.8% +/- 0.1% (SEM) of total faecal bile acids. In addition to a detailed description of the methodology the effect of antibiotics on faecal bile acid profiles is ...Continue Reading

References

Jan 16, 1976·Clinica Chimica Acta; International Journal of Clinical Chemistry·O J Roseleur, C M van Gent
Aug 1, 1979·Clinica Chimica Acta; International Journal of Clinical Chemistry·A CantaforaL Capocaccia
Feb 1, 1976·Journal of the National Cancer Institute·B S ReddyE L Wynder
Jun 1, 1973·Journal of the National Cancer Institute·B S Reddy, E L Wynder
Jun 1, 1971·The Journal of Pathology·M J Hill, V C Aries
Aug 1, 1969·The British Journal of Nutrition·B E Gustafsson, A Norman
Apr 1, 1981·Analytical Biochemistry·M A IslamB I Cohen
Jun 1, 1981·Steroids·B I CohenN B Javitt
May 1, 1981·Journal of Steroid Biochemistry·T FotsisJ Sjövall
Jan 1, 1964·The British Journal of Nutrition·A NORMAN

❮ Previous
Next ❯

Citations

Dec 17, 2009·Journal of Lipid Research·William J Griffiths, Jan Sjövall
Mar 5, 2016·Gut Microbes·Jason M RidlonPhillip B Hylemon
Jul 26, 2007·Journal of Applied Microbiology·M C CasiraghiL Villa
Nov 1, 1988·Biomedical Chromatography : BMC·J M Street, K D Setchell
Sep 12, 2014·Journal of Lipid Research·Paul A Dawson, Saul J Karpen
Oct 29, 2008·Journal of Pediatric Gastroenterology and Nutrition·Alan F HofmannDale A Chatfield
Jan 1, 1997·Scandinavian Journal of Gastroenterology. Supplement·R W Owen
Jan 1, 1990·Journal of Pharmaceutical and Biomedical Analysis·S Scalia
Sep 16, 1992·Journal of Chromatography·T M de KokJ C Kleinjans
May 29, 1999·Journal of Chromatography. B, Biomedical Sciences and Applications·S Chaudhury, M F Chaplin

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.