Nov 1, 1984

Multicomponent RNA plant virus infection derived from cloned viral cDNA

Proceedings of the National Academy of Sciences of the United States of America
P AhlquistL S Loesch-Fries

Abstract

In vitro transcripts from mixtures of appropriate brome mosaic virus (BMV) cDNA clones are infectious when inoculated onto barley plants. Infectivity depends on in vitro transcription and on the presence of transcripts from clones of all three BMV genetic components. Infectivity is destroyed by RNase after transcription, but it is insensitive to RNase before or to DNase after transcription. Virion RNAs from plants infected with cDNA transcripts hybridize to BMV-specific probes and coelectrophorese with virion RNAs propagated from conventional inoculum. Direct RNA sequencing shows that a deletion in the noncoding region of one infectious BMV clone is preserved in viral RNA from plants systemically infected with transcript mixtures representing that clone.

Mentioned in this Paper

Deoxyribonuclease I
Sequence Determinations, RNA
Virion
Gene Deletion Abnormality
Biologic Preservation
Hordeum
Deletion Mutation
Pancreatic ribonuclease
Hordeum vulgare
RNA, Viral

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